Potter P C, Van Wyk L, White D, Dakers B S, Chung F Z, Dowdle E B
Department of Clinical Science and Immunology, University of Cape Town, Observatory, South Africa.
Clin Exp Allergy. 1993 Mar;23(3):219-25. doi: 10.1111/j.1365-2222.1993.tb00885.x.
Using guinea-pig lung membranes and cloned human beta 2-receptor adrenergic receptors the effects of whole serum, plasma, purified immunoglobulins and cellular activation products on beta 2-adrenergic receptor ligand binding and function were investigated. Sera from 24 non-asthmatic subjects and 115 asthmatics in different clinical categories were studied. There were no significant differences between antagonist ([125I] cyanopindolol) inhibition mediated by serum, plasma or by purified IgG when the asthmatics were compared with non-asthmatics. There was also no inhibition of 10(-6) M isoproterenol stimulated cAMP release from L cells expressing human beta 2-adrenergic receptors, by plasma, DEAE purified IgG fractions from asthmatics and non-asthmatics, or by products of activated platelets or lymphocytes. Since we have no evidence that immunoglobulins from asthmatic subjects exert functional inhibition of human beta 2-adrenergic receptors we conclude that autoantibodies to the beta 2-adrenergic receptors do not play an important functional role in the pathophysiology of asthma.
利用豚鼠肺膜和克隆的人β2 -肾上腺素能受体，研究了全血清、血浆、纯化免疫球蛋白和细胞活化产物对β2 -肾上腺素能受体配体结合及功能的影响。研究了来自24名非哮喘患者和115名不同临床类型哮喘患者的血清。将哮喘患者与非哮喘患者进行比较时，血清、血浆或纯化IgG介导的拮抗剂（[125I]氰胍心安）抑制作用无显著差异。血浆、哮喘患者和非哮喘患者经DEAE纯化的IgG组分、活化血小板或淋巴细胞的产物，对10(-6) M异丙肾上腺素刺激表达人β2 -肾上腺素能受体的L细胞释放cAMP也无抑制作用。由于我们没有证据表明哮喘患者的免疫球蛋白对人β2 -肾上腺素能受体发挥功能抑制作用，因此我们得出结论，β2 -肾上腺素能受体自身抗体在哮喘的病理生理学中不发挥重要的功能作用。
