The diffusional water permeability (P) of mouse and rat red blood cell (RBC) membrane has been monitored by a doping nuclear magnetic resonance (NMR) technique on control cells and following exposure to various concentrations of p-chloromercuribenzene sulfonate (PCMBS). 2. There were no significant differences in the water permeability of mouse and rat RBCs, the values of P being around 6 x 10(-3)/sec at 20 degrees C and 11 x 10(-3) cm/sec at 37 degrees C. 3. Systematic studies of the effects of PCMBS on water diffusion indicated that the maximal inhibition is reached in 60 min at 20 degrees C with 1 mM PCMBS for the mouse RBCs and with 2 mM PCMBS for the rat RBCs. 4. The values of maximal inhibition ranged from 55-57% at 37 degrees C and reached values around 70% at 10 degrees C. 5. The degree of inhibition increased as the temperature of measurement decreased, regardless of PCMBS concentration and incubation time. 6. The basal permeability to water of mouse RBCs was estimated as 1.8 x 10(-3) cm/sec at 20 degrees C and 4.6 x 10(-3) cm/sec at 37 degrees C, and that of rat RBCs as 2.2 x 10(-3) cm/sec at 20 degrees C and 4.2 x 10(-3) cm/sec at 37 degrees C. 7. In both species the activation energy was around 27 kJ/mol and reached values over 40 kJ/mol after incubation with PCMBS in the conditions of maximal inhibition of water diffusion.
摘要
通过掺杂核磁共振（NMR）技术，对对照细胞以及暴露于不同浓度对氯汞苯磺酸盐（PCMBS）后的小鼠和大鼠红细胞（RBC）膜的扩散水渗透率（P）进行了监测。2. 小鼠和大鼠红细胞的水渗透率没有显著差异，在20℃时P值约为6×10⁻³/秒，在37℃时为11×10⁻³厘米/秒。3. 对PCMBS对水扩散影响的系统研究表明，在20℃下，1 mM PCMBS作用于小鼠红细胞、2 mM PCMBS作用于大鼠红细胞时，60分钟内可达到最大抑制。4. 在37℃时最大抑制值范围为55 - 57%，在10℃时达到约70%。5. 无论PCMBS浓度和孵育时间如何，抑制程度随着测量温度的降低而增加。6. 小鼠红细胞在20℃时的基础水渗透率估计为1.8×10⁻³厘米/秒，在37℃时为4.6×10⁻³厘米/秒；大鼠红细胞在20℃时为2.2×10⁻³厘米/秒，在37℃时为4.2×10⁻³厘米/秒。7. 在两个物种中，活化能约为27 kJ/mol，在水扩散最大抑制条件下与PCMBS孵育后达到超过40 kJ/mol的值。
