Ryberg D, Heimdal K, Fosså S D, Børresen A L, Haugen A
Department of Toxicology, National Institute of Occupational Health, Oslo, Norway.
Int J Cancer. 1993 Apr 1;53(6):938-40. doi: 10.1002/ijc.2910530612.
We have examined the restriction-fragment-length polymorphism (RFLP) of the VNTR region flanking the Ha-ras1 gene in genomic DNA from 348 testicular cancer patients and 343 healthy controls. When DNA samples were digested with MspI/HpaII, Southern blot analysis revealed 24 and 15 different alleles in the cancer and the control group respectively. The frequency of rare alleles among cases was significantly higher than in controls. Patients with bilateral cancer and patients with unilateral cancer below 20 years had significantly higher incidence of rare alleles than patients with unilateral cancer older than 20 years.
我们检测了348例睾丸癌患者和343例健康对照者基因组DNA中Ha-ras1基因侧翼VNTR区域的限制性片段长度多态性（RFLP）。当用MspI/HpaII消化DNA样本时，Southern印迹分析显示癌症组和对照组分别有24个和15个不同的等位基因。病例组中稀有等位基因的频率显著高于对照组。双侧癌症患者和20岁以下的单侧癌症患者中稀有等位基因的发生率显著高于20岁以上的单侧癌症患者。
