Mandl C W, Holzmann H, Kunz C, Heinz F X
Institute of Virology, Vienna, Austria.
Virology. 1993 May;194(1):173-84. doi: 10.1006/viro.1993.1247.
The complete nucleotide sequence of the positive-stranded RNA genome of the tick-borne flavivirus Powassan (10,839 nucleotides) was elucidated and the amino acid sequence of all viral proteins was derived. Based on this sequence as well as serological data, Powassan virus represents the most divergent member of the tick-borne serocomplex within the genus flaviviruses, family Flaviviridae. The primary nucleotide sequence and potential RNA secondary structures of the Powassan virus genome as well as the protein sequences and the reactivities of the virion with a panel of monoclonal antibodies were compared to other tick-borne and mosquito-borne flaviviruses. These analyses corroborated significant differences between tick-borne and mosquito-borne flaviviruses, but also emphasized structural elements that are conserved among both vector groups. The comparisons among tick-borne flaviviruses revealed conserved sequence elements that might represent important determinants of the tick-borne flavivirus phenotype.
蜱传黄病毒波瓦桑病毒（Powassan）正链RNA基因组的完整核苷酸序列（10,839个核苷酸）已被阐明，并且推导了所有病毒蛋白的氨基酸序列。基于该序列以及血清学数据，波瓦桑病毒是黄病毒科黄病毒属中蜱传血清复合体中最具差异性的成员。将波瓦桑病毒基因组的一级核苷酸序列和潜在的RNA二级结构以及病毒粒子的蛋白质序列和与一组单克隆抗体的反应性与其他蜱传和蚊传黄病毒进行了比较。这些分析证实了蜱传和蚊传黄病毒之间的显著差异，但也强调了在两个载体组中保守的结构元件。蜱传黄病毒之间的比较揭示了保守的序列元件，这些元件可能代表蜱传黄病毒表型的重要决定因素。
