Moore K G, Donadio A C, Sartorelli A C
Department of Pharmacology, Yale University School of Medicine, New Haven CT 06510.
Biochem Biophys Res Commun. 1993 Apr 30;192(2):381-5. doi: 10.1006/bbrc.1993.1426.
The presence of type I transglutaminase was determined in the neoplastic human keratinocyte line SqCC/Y1 and in normal human epidermal keratinocytes (NHEK) by an in situ radioimmunoassay which corresponded directly with the measurement of type I transglutaminase enzymatic activity. Dexamethasone induced differentiation of SqCC/Y1 cells caused a marked increase in transglutaminase immunoreactivity and enzymatic activity over non-steroid treated cells in a concentration-related and a time-related fashion. Retinoic acid suppressed the dexamethasone induced increase in type I transglutaminase immunoreactivity in differentiating SqCC/Y1 cells. The type I transglutaminase radioimmunoassay should be useful in studies focusing on the regulation of transglutaminase activity in normal and neoplastic keratinocytes, and for rapidly screening agents for their effects on squamous cell differentiation.
通过原位放射免疫测定法，在人肿瘤性角质形成细胞系SqCC/Y1和正常人表皮角质形成细胞（NHEK）中测定了I型转谷氨酰胺酶的存在情况，该方法与I型转谷氨酰胺酶酶活性的测量直接相关。地塞米松诱导SqCC/Y1细胞分化，导致转谷氨酰胺酶免疫反应性和酶活性相对于未用类固醇处理的细胞以浓度和时间相关的方式显著增加。维甲酸抑制了地塞米松诱导的分化SqCC/Y1细胞中I型转谷氨酰胺酶免疫反应性的增加。I型转谷氨酰胺酶放射免疫测定法在聚焦于正常和肿瘤性角质形成细胞中转谷氨酰胺酶活性调节的研究中应是有用的，并且可用于快速筛选对鳞状细胞分化有影响的药物。
