人类II型肌苷酸脱氢酶基因（IMPDH2）的染色体定位与结构

Chromosomal localization and structure of the human type II IMP dehydrogenase gene (IMPDH2).

作者信息

Glesne D, Collart F, Varkony T, Drabkin H, Huberman E

机构信息

Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.

出版信息

Genomics. 1993 Apr;16(1):274-7. doi: 10.1006/geno.1993.1177.

DOI:10.1006/geno.1993.1177

PMID:8098009

Abstract

We determined the chromosomal localization and structure of the gene encoding human type II inosine 5'-monophosphate dehydrogenase (IMPDH, EC 1.1.1.205), an enzyme associated with cellular proliferation, malignant transformation, and differentiation. Using polymerase chain reaction (PCR) primers specific for type II IMPDH, we screened a panel of human-Chinese hamster cell somatic hybrids and a separate deletion panel of chromosome 3 hybrids and localized the gene to 3p21.2-->p24.2. Two overlapping yeast artificial chromosome clones containing the full gene for type II IMPDH (IMPDH2) were isolated, and a physical map of 117 kb of human genomic DNA in this region of chromosome 3 was constructed. The gene for type II IMPDH was localized and oriented on this map and found to span no more than 12.5 kb.

摘要

我们确定了编码人II型肌苷5'-单磷酸脱氢酶（IMPDH，EC 1.1.1.205）的基因的染色体定位和结构，该酶与细胞增殖、恶性转化及分化相关。利用针对II型IMPDH的特异性聚合酶链反应（PCR）引物，我们筛选了一组人-中国仓鼠体细胞杂种以及一个单独的3号染色体杂种缺失组，并将该基因定位到3p21.2→p24.2。分离出了两个包含II型IMPDH（IMPDH2）完整基因的重叠酵母人工染色体克隆，并构建了3号染色体该区域117 kb人类基因组DNA的物理图谱。II型IMPDH基因在该图谱上进行了定位和定向，发现其跨度不超过12.5 kb。