Rommens C M, Munyikwa T R, Overduin B, Nijkamp H J, Hille J
Department of Genetics, Free University, Amsterdam, Netherlands.
Plant Mol Biol. 1993 Mar;21(6):1109-19. doi: 10.1007/BF00023607.
Several aspects of transposition of an in vitro modified Ds element are described. This Ds element, designated Ds-r, is equipped with bacterial plasmid sequences and can, therefore, be rescued from the plant genome. Our results indicate that the Ds-r element has a 'late' timing of transposition from T-DNAs. This feature of the element might be advantageous for tagging experiments because it leads to independently transposed germinally transmitted elements. Furthermore, it is shown that Ds-r transposition generates clusters of insertions, indicating that 'genes to be tagged' should be located in genomic regions covered by insertions.
本文描述了体外修饰的Ds元件转座的几个方面。这种Ds元件被命名为Ds-r，它带有细菌质粒序列，因此可以从植物基因组中拯救出来。我们的结果表明，Ds-r元件从T-DNA进行转座的时间较“晚”。该元件的这一特性可能对标签实验有利，因为它会产生独立转座的可通过有性生殖传递的元件。此外，研究表明Ds-r转座会产生插入簇，这表明“待标记的基因”应位于插入所覆盖的基因组区域。
