来自T-DNA的Ac转座可在番茄基因组中产生连锁和非连锁的插入簇。
Ac transposition from a T-DNA can generate linked and unlinked clusters of insertions in the tomato genome.
作者信息
Osborne B I, Corr C A, Prince J P, Hehl R, Tanksley S D, McCormick S, Baker B
机构信息
Plant Gene Expression Center, Albany, California 94710.
出版信息
Genetics. 1991 Nov;129(3):833-44. doi: 10.1093/genetics/129.3.833.
Abstract
We have investigated the distribution of transposed Acs in the tomato genome. Our approach has been to clone the regions flanking the T-DNAs and transposed Acs from two transgenic lines of tomato and place these sequences on the tomato restriction fragment length polymorphism (RFLP) map. The distribution of transposed Acs around the T-DNA and at locations unlinked to the T-DNA indicates that Ac transposes to linked and unlinked sites in tomato as it does in maize. The structure and terminal sequence of these cloned elements shows that Ac remains intact after transposition. We discuss these results and their bearing on gene tagging strategies using Ac and Ds.
摘要
我们研究了转座的Acs在番茄基因组中的分布情况。我们采用的方法是，从两个番茄转基因系中克隆T-DNAs侧翼区域和转座的Acs，并将这些序列定位到番茄限制性片段长度多态性（RFLP）图谱上。转座的Acs在T-DNA周围以及与T-DNA不连锁的位置上的分布情况表明，Acs在番茄中如同在玉米中一样，会转座到连锁和不连锁的位点。这些克隆元件的结构和末端序列显示，Acs在转座后仍保持完整。我们讨论了这些结果及其对使用Acs和Ds进行基因标签策略的影响。
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