• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Identification of chaperonin particles in mammalian brain cytosol and of T-complex polypeptide 1 as one of their components.

作者信息

Roobol A, Carden M J

机构信息

Biological Laboratory, University of Kent, Canterbury, England.

出版信息

J Neurochem. 1993 Jun;60(6):2327-30. doi: 10.1111/j.1471-4159.1993.tb03524.x.

DOI:10.1111/j.1471-4159.1993.tb03524.x
PMID:8098357
Abstract

An approximately 950-kDa heteromeric particle was purified from guinea-pig and rat brain by sucrose gradient fractionation of post-mitochondrial supernatants. Further purification, by affinity chromatography on ATP-Sepharose and anion exchange FPLC on MonoQ, yielded a particle with typical chaperonin ultrastructure. One of the component polypeptides was recognized by a monoclonal antibody to murine T-complex polypeptide 1. Brain cytosolic chaperonin particles formed a binary complex with unfolded tubulin subunits. The polypeptide compositions of the cytosolic chaperonin particles appeared very similar between brain and testicular tissues of the same animal, but differed subtly between the guinea-pig and rat.

摘要

相似文献

1
Identification of chaperonin particles in mammalian brain cytosol and of T-complex polypeptide 1 as one of their components.
J Neurochem. 1993 Jun;60(6):2327-30. doi: 10.1111/j.1471-4159.1993.tb03524.x.
2
Eukaryotic cytosolic chaperonin contains t-complex polypeptide 1 and seven related subunits.真核细胞胞质伴侣蛋白包含t-复合体多肽1和七个相关亚基。
Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11975-9. doi: 10.1073/pnas.90.24.11975.
3
Mammalian cytosolic chaperonin.
Methods Enzymol. 1998;290:230-41. doi: 10.1016/s0076-6879(98)90022-2.
4
Purification of the cytosolic chaperonin TRiC from bovine testis.从牛睾丸中纯化胞质伴侣蛋白TRiC
Methods Mol Biol. 2000;140:153-60. doi: 10.1385/1-59259-061-6:153.
5
The t-complex polypeptide 1 complex is a chaperonin for tubulin and actin in vivo.t-复合体多肽1复合体在体内是微管蛋白和肌动蛋白的伴侣蛋白。
Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9422-6. doi: 10.1073/pnas.90.20.9422.
6
gamma-Tubulin in mammalian cells: the centrosomal and the cytosolic forms.哺乳动物细胞中的γ-微管蛋白：中心体形式和胞质形式
J Cell Sci. 1996 Apr;109 ( Pt 4):875-87. doi: 10.1242/jcs.109.4.875.
7
Cytoplasmic chaperonin complexes enter neurites developing in vitro and differ in subunit composition within single cells.
J Cell Sci. 1995 Apr;108 ( Pt 4):1477-88. doi: 10.1242/jcs.108.4.1477.
8
Newly-synthesized beta-tubulin demonstrates domain-specific interactions with the cytosolic chaperonin.新合成的β-微管蛋白表现出与胞质伴侣蛋白的结构域特异性相互作用。
Biochemistry. 1996 Dec 10;35(49):15870-82. doi: 10.1021/bi961114j.
9
Brain t-complex polypeptide 1 (TCP- 1) related to its natural substrate beta1 tubulin is decreased in Alzheimer's disease.
Life Sci. 2001 Jun 8;69(3):263-70. doi: 10.1016/s0024-3205(01)01126-2.
10
Tissue-specific subunit of the mouse cytosolic chaperonin-containing TCP-1.小鼠含TCP-1的胞质伴侣蛋白的组织特异性亚基。
FEBS Lett. 1997 Jan 27;402(1):53-6. doi: 10.1016/s0014-5793(96)01501-3.

引用本文的文献

1
Eukaryotic chaperonin containing T-complex polypeptide 1 interacts with filamentous actin and reduces the initial rate of actin polymerization in vitro.含有T复合物多肽1的真核伴侣蛋白与丝状肌动蛋白相互作用，并在体外降低肌动蛋白聚合的初始速率。
Cell Stress Chaperones. 2002 Jul;7(3):235-42. doi: 10.1379/1466-1268(2002)007<0235:ecctcp>2.0.co;2.
2
The third member of the Tetrahymena CCT subunit gene family, TpCCT alpha, encodes a component of the hetero-oligomeric chaperonin complex.嗜热四膜虫CCT亚基基因家族的第三个成员TpCCTα，编码异源寡聚伴侣蛋白复合体的一个组分。
Biochem J. 1997 Aug 15;326 ( Pt 1)(Pt 1):21-9. doi: 10.1042/bj3260021.
3
Antisense oligonucleotide to the 70-kDa heat shock cognate protein inhibits synthesis of myelin basic protein.
Neurochem Res. 1996 Apr;21(4):417-22. doi: 10.1007/BF02527705.