Michálek A, Marcaník J
Vet Med (Praha). 1975 Apr;20(4):199-206.
The authors describe selected methods of agar-gel electrophoresis enabling the qualitative and quantitative study of LDH isoenzymes in animal serum. Isoenzyme values: horse LDH-1 33.0%, LDH-2 27.9%, LDH-3 24.3%, LDH-4 9.6%, LDH-5 5.2%; sheep LDH-1 57%, LDH-2 10.8%, LDH-3 21.6%, LDH-4 5.2%, LDH-5 5.4%; cow LDH-1 45.9%, LDH-2 25.8%, LDH-3 15%, LDH-4 7%, LDH-5 6.8%; pig LDH-1 44.4%, LDH-2 24.9%, LDH-3 13.6%, LDH-4 8.8%, LDH-5 8.3%. Under the same conditions, the lowest degree of heterogeneity was found in the enzyme of sheep serum.
作者描述了琼脂凝胶电泳的选定方法，这些方法能够对动物血清中的乳酸脱氢酶同工酶进行定性和定量研究。同工酶值：马的乳酸脱氢酶-1为33.0%，乳酸脱氢酶-2为27.9%，乳酸脱氢酶-3为24.3%，乳酸脱氢酶-4为9.6%，乳酸脱氢酶-5为5.2%；绵羊的乳酸脱氢酶-1为57%，乳酸脱氢酶-2为10.8%，乳酸脱氢酶-3为21.6%，乳酸脱氢酶-4为5.2%，乳酸脱氢酶-5为5.4%；牛的乳酸脱氢酶-1为45.9%，乳酸脱氢酶-2为25.8%，乳酸脱氢酶-3为15%，乳酸脱氢酶-4为7%，乳酸脱氢酶-5为6.8%；猪的乳酸脱氢酶-1为44.4%，乳酸脱氢酶-2为24.9%，乳酸脱氢酶-3为13.6%，乳酸脱氢酶-4为8.8%，乳酸脱氢酶-5为8.3%。在相同条件下，绵羊血清酶的异质性程度最低。
