Ryan A M, Womack J E
Department of Veterinary Pathobiology, Texas A&M University, College Station 77843-4467.
Anim Genet. 1993 Feb;24(1):23-6. doi: 10.1111/j.1365-2052.1993.tb00914.x.
Brains affected by the progressive neurological disease bovine spongiform encephalopathy (BSE) contain scrapie-associated fibrils and the protease-resistant isoform of prion protein. The gene encoding the normal host prion protein (PRNP) has been mapped to human chromosome 20 and mouse chromosome 2 with the hamster cDNA probe pEA974. Using this probe and a panel of bovine-rodent hybrid somatic cells, we have mapped PRNP to bovine syntenic group U11 (100% concordancy). PRNP restriction fragment length polymorphisms (RFLPs) were detected with five of six enzymes (BglII, EcoRI, HindIII, MspI and TaqI) in sheep, in contrast to one of 16 enzymes (HincII) in cattle. Codominant segregation of the bovine HincII RFLP was demonstrated in six backcross pedigrees. While PRNP RFLPs are tightly linked to scrapie incubation period, and consequently susceptibility or resistance to disease in rodents and sheep, the relationship between the PRNP RFLPs and BSE incubation period has not been determined.
受进行性神经疾病牛海绵状脑病（BSE）影响的大脑含有羊瘙痒病相关纤维和抗蛋白酶的朊病毒蛋白异构体。利用仓鼠cDNA探针pEA974，已将编码正常宿主朊病毒蛋白（PRNP）的基因定位到人类20号染色体和小鼠2号染色体上。使用该探针和一组牛-啮齿动物杂交体细胞，我们已将PRNP定位到牛同线群U11（一致性为100%）。在绵羊中，用六种酶中的五种（BglII、EcoRI、HindIII、MspI和TaqI）检测到PRNP限制性片段长度多态性（RFLP），而在牛中16种酶中只有一种（HincII）能检测到。在六个回交家系中证明了牛HincII RFLP的共显性分离。虽然PRNP RFLP与羊瘙痒病潜伏期紧密相关，因此与啮齿动物和绵羊对疾病的易感性或抗性相关，但PRNP RFLP与BSE潜伏期之间的关系尚未确定。
