牛、河水牛、绵羊和山羊染色体上朊病毒蛋白基因（PRNP）的比较荧光原位杂交图谱分析。

Comparative FISH-mapping of the prion protein gene (PRNP) on cattle, river buffalo, sheep and goat chromosomes.

作者信息

Iannuzzi L, Palomba R, Di Meo G P, Perucatti A, Ferrara L

机构信息

National Research Council (CNR), I.A.B.B.A.M., Naples, Italy.

出版信息

Cytogenet Cell Genet. 1998;81(3-4):202-4. doi: 10.1159/000015030.

DOI:10.1159/000015030

PMID:9730603

Abstract

Comparative FISH-mapping of the prion protein gene (PRNP) was performed on cattle (BTA), river buffalo (BBU), sheep (OAR) and goat (CHI) chromosomes using a PCR-product as a probe and R-banding. PRNP was mapped to BTA13q17, BBU14q15, OAR13q15 and CHI13q15 according to standard nomenclatures. These chromosomes and bands were homoeologous among the four species, confirming the high degree of gene and chromosome banding conservation among bovids. Furthermore, the assignment of PRNP to river buffalo and goat chromosomes allowed us to indirectly assign the bovine syntenic group U11 to specific chromosomes, since it is the first in situ localization on BBU14 and CHI13.

摘要

利用聚合酶链反应（PCR）产物作为探针并结合R显带技术，对牛（BTA）、河水牛（BBU）、绵羊（OAR）和山羊（CHI）的染色体进行了朊病毒蛋白基因（PRNP）的比较荧光原位杂交（FISH）定位。根据标准命名法，PRNP被定位到BTA13q17、BBU14q15、OAR13q15和CHI13q15。这四条染色体及其带区在四个物种间是同源的，证实了牛科动物之间基因和染色体带型的高度保守性。此外，将PRNP定位到河水牛和山羊染色体上，使我们能够间接将牛的同线群U11定位到特定染色体上，因为这是该同线群在BBU14和CHI13上的首次原位定位。