Metge K, Steube K, Uphoff C C, Gignac S M, Drexler H G
DSM-German Collection of Microorganisms and Cell Cultures, Department of Human and Animal Cell Cultures, Braunschweig.
Leukemia. 1993 Jun;7(6):896-7.
We examined the expression of the mdr1 gene in 90 human tumor cell lines of which 79 were derived from leukemia or lymphoma samples. Using Northern blot analysis and the mdr1.1 probe, only three cell lines, KB-V1 (a positive control), EM-2, A-704, displayed mdr1 mRNA expression. Immunocytochemical APAAP staining of these three and 15 other cell lines with the anti-p170 glycoprotein monoclonal antibody JSB-1 was negative in all but the KB-V1 cells. In contrast to the results seen in freshly explanted neoplastic cells, the mdr1 gene does not seem to be functionally expressed in the majority of unmanipulated and unselected, continuous human tumor cell lines.
我们检测了90种人类肿瘤细胞系中mdr1基因的表达情况，其中79种源自白血病或淋巴瘤样本。使用Northern印迹分析和mdr1.1探针，只有三种细胞系，即KB-V1（阳性对照）、EM-2、A-704，显示出mdr1 mRNA表达。用抗p170糖蛋白单克隆抗体JSB-1对这三种细胞系以及另外15种细胞系进行免疫细胞化学APAAP染色，除KB-V1细胞外，其余均为阴性。与新鲜分离的肿瘤细胞中的结果相反，mdr1基因似乎在大多数未经处理和未筛选的连续人类肿瘤细胞系中未功能性表达。
