McDowell H, Peuchmaur M, Dominici C, Flamant F
Department of Paediatrics, Institut Gustave Roussy, Villejuif, France.
Anticancer Res. 1993 Sep-Oct;13(5C):1863-6.
Twenty-four malignant mesenchymal tumour specimens were analysed for human multidrug resistance (MDRI) gene transcript levels using Northern and slot blot techniques. The presence of P-glycoprotein was assessed in 12 of the 24 samples by immunohistochemistry using the monoclonal antibody (MAb) C219. Increased MDRI transcript levels were found in 2 (8.3%), while, using immunohistochemistry, 2 samples were positive and 3 faintly positive (41.6%). Overall, elevated P-glycoprotein or MDRI transcript levels were found in tumours of 6 patients, 3 of whom are dead. The relationship to MDRI expression and subsequent resistance to chemotherapy has to be established.
使用Northern印迹法和狭缝印迹法对24份恶性间充质肿瘤标本进行了人类多药耐药（MDR1）基因转录水平分析。通过使用单克隆抗体（MAb）C219的免疫组织化学方法，对24份样本中的12份进行了P-糖蛋白检测。在2份样本（8.3%）中发现MDR1转录水平升高，而通过免疫组织化学检测，2份样本呈阳性，3份样本弱阳性（41.6%）。总体而言，在6例患者的肿瘤中发现P-糖蛋白或MDR1转录水平升高，其中3例患者已死亡。MDR1表达与后续化疗耐药之间的关系有待确定。
