Karásek J
Virchows Arch B Cell Pathol. 1975 Sep 11;18(4):337-46. doi: 10.1007/BF02889260.
The ultrastructural changes of extranucleolar nuclear ribonucleoprotein structures during autolysis of normal liver cells were studied by means of usual and cytochemical procedures. The results revealed that the earliest changes were characterized by the fragmentation of the perichromatin fibrils and disappearance of the perichromatin granules. This was followed by the formation of numerous dense granules among the altered perichromatin fibrils and by the disappearance of dense fibrillar structures from the remaining interchromatin areas. In the later stages of autolysis the altered perichromatin fibrils loosed their density and the dense granules located among them disappeared. In addition, fine filamentous and cross-striated bodies appeared in the nucleoplasm as well as in the cytoplasm of some hepatocytes. The results obtained by the EDTA treatment and digestion of the sections with Pronase and RNA-ase indicated that the altered perichromatin fibrils contained ribonucleoprotein which together with that of the perichromatin granules represent nuclear components most sensitive to the autolysis. The chemical nature of the dense granules located among the altered perichromatin fibrils was different from that of the latter as well as from that of the nuclear microspherules [6]. The dense fine filamentous and cross-striated nucleoplasmic and cytoplasmic bodies are considered to represent a nonspecific structural phenomenon accompanying regressive cellular alterations since these bodies were present only in the later stages of autolysis.
采用常规及细胞化学方法，研究了正常肝细胞自溶过程中核仁外核糖核蛋白结构的超微结构变化。结果显示，最早的变化特征为染色质周缘纤维的断裂和染色质周缘颗粒的消失。随后，在改变的染色质周缘纤维之间形成大量致密颗粒，剩余染色质间区域的致密纤维结构消失。在自溶后期，改变的染色质周缘纤维密度降低，位于其中的致密颗粒消失。此外，在一些肝细胞的核质以及细胞质中出现了细丝状和横纹状小体。通过乙二胺四乙酸（EDTA）处理以及用链霉蛋白酶和核糖核酸酶消化切片所获得的结果表明，改变的染色质周缘纤维含有核糖核蛋白，其与染色质周缘颗粒的核糖核蛋白一起代表了对自溶最敏感的核成分。位于改变的染色质周缘纤维之间的致密颗粒的化学性质与后者以及核小球的化学性质不同[6]。致密的细丝状和横纹状核质及细胞质小体被认为代表了伴随细胞退行性改变的一种非特异性结构现象，因为这些小体仅出现在自溶后期。
