Smetana K, Daskal I, Busch H
Histochemistry. 1979 Jul 11;61(3):327-34. doi: 10.1007/BF00508454.
The perichromatin granules were studied in hepatocytes of experimental rats injected with cycloheximide because the increased number of these nuclear components after such treatment facilitated their cytochemical investigation. Most perichromatin granules were sensitive to the digestion with pepsin and ribonuclease. In contrast, small population of perichromatin granules was resistent to such digestion under conditions which remove known RNA containing components such as ribosomes, nucleolar RNP components and interchromatin granules. The size of these resistent perichromatin granules was reduced and they consisted of filaments the width of which was similar to that of filaments in the chromatin. Moreover, a small population of perichromatin granules was sensitive to the digestion with pepsin and deoxyribonuclease. The size of these granules was only slightly reduced. All these observations indicate that most perichromatin granules contain the RNA and some the DNA. A possibility also exists that the perichromatin granules might contain both RNA and DNA but in various proportions. In addition, partial digestion with pepsin followed by a complete digestion with ribonuclease and deoxyribonuclease removed perichromatin granules as well as other nucleoprotein structures. On the other hand, such digestion facilitated the visualization of the nuclear and cytoplasmic skeleton (matrix) in situ.
对注射放线菌酮的实验大鼠肝细胞中的染色质旁颗粒进行了研究，因为这种处理后这些核成分数量增加，便于进行细胞化学研究。大多数染色质旁颗粒对胃蛋白酶和核糖核酸酶消化敏感。相比之下，在去除已知含RNA成分（如核糖体、核仁核糖核蛋白成分和染色质间颗粒）的条件下，一小部分染色质旁颗粒对这种消化具有抗性。这些抗性染色质旁颗粒的大小减小，它们由细丝组成，细丝宽度与染色质中的细丝相似。此外，一小部分染色质旁颗粒对胃蛋白酶和脱氧核糖核酸酶消化敏感。这些颗粒的大小仅略有减小。所有这些观察结果表明，大多数染色质旁颗粒含有RNA，一些含有DNA。也有可能染色质旁颗粒可能同时含有RNA和DNA，但比例不同。此外，先用胃蛋白酶部分消化，再用核糖核酸酶和脱氧核糖核酸酶完全消化，可去除染色质旁颗粒以及其他核蛋白结构。另一方面，这种消化有助于原位观察核和细胞质骨架（基质）。
