Oster T, Thioudellet C, Chevalot I, Masson C, Wellman M, Marc A, Siest G
Centre du Médicament, URA CNRS 597, Nancy, France.
Biochem Biophys Res Commun. 1993 May 28;193(1):406-12. doi: 10.1006/bbrc.1993.1638.
Sodium butyrate was used to enhance biosynthesis rates of recombinant human gamma-glutamyl transferase (GGT) expressed under the control of the SV40 or the cytomegalovirus immediate early promoter, respectively, in transfected V79 and CHO Chinese hamster cell lines. Maximal induction of GGT specific activity in butyrate-treated cells ranged from 3 to 5-fold and resulted from a strong increase in the GGT mRNA ratio. We also observed that maximal transcription level in V79 cells occurred within 12 hr of treatment, whilst the cell proliferation was transiently arrested. Despite its processing requirements, induced GGT exhibited unchanged catalytic and physico-chemical features relative to human serum or hepatoma enzyme, thus appearing as an excellent model for further studies on human GGT.
丁酸钠用于提高在SV40或巨细胞病毒立即早期启动子控制下分别在转染的V79和CHO中国仓鼠细胞系中表达的重组人γ-谷氨酰转移酶（GGT）的生物合成速率。丁酸钠处理的细胞中GGT比活性的最大诱导范围为3至5倍，这是由于GGT mRNA比例的显著增加所致。我们还观察到，V79细胞中的最大转录水平在处理后12小时内出现，而细胞增殖则短暂停滞。尽管有其加工要求，但诱导的GGT相对于人血清或肝癌酶表现出不变的催化和物理化学特性，因此成为进一步研究人GGT的极佳模型。
