Treichel R S
Department of Biology, Oberlin College, OH 44074-1082.
Leuk Res. 1993 Jun;17(6):491-9. doi: 10.1016/0145-2126(93)90124-4.
Nine drug-resistant variants of human leukemic/lymphoma cell lines were evaluated for the expression of antigens which are commonly targeted in antibody-dependent purging protocols used for autologous bone marrow transplantation. Flow cytometry revealed that most drug-resistant variants differ in antigen expression from parental lines. Both epipodophyllotoxin-selected T-ALL variants tested expressed lower levels of CD5 than parental counterparts. In contrast, other antigens (CD7, CD3, CD4, CD8, CD38, CD9, CD10, CD24, CD19, CD20) were not consistently altered in drug-resistant variants. Drug-resistant cells were lysed as effectively as drug-sensitive cells by murine antibody and baby rabbit complement, but only when particular monoclonal antibodies or cocktails of monoclonal antibodies were utilized. Two of the nine drug-resistant variants proved to be more resistant than parental cells to antibody-dependent cell-mediated cytolysis (ADCC).
对九种人类白血病/淋巴瘤细胞系的耐药变体进行了评估，以检测那些在用于自体骨髓移植的抗体依赖净化方案中通常作为靶点的抗原的表达情况。流式细胞术显示，大多数耐药变体在抗原表达上与亲代细胞系不同。所检测的两种经表鬼臼毒素筛选的T-ALL变体表达的CD5水平低于亲代细胞。相比之下，其他抗原（CD7、CD3、CD4、CD8、CD38、CD9、CD10、CD24、CD19、CD20）在耐药变体中并未持续发生改变。耐药细胞与药物敏感细胞一样能被鼠抗体和兔补体有效裂解，但前提是使用特定的单克隆抗体或单克隆抗体组合。九个耐药变体中的两个被证明比亲代细胞对抗体依赖的细胞介导的细胞毒性作用（ADCC）更具抗性。
