Shibata S, Watanabe S
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Neurosci Lett. 1993 Mar 19;151(2):138-41. doi: 10.1016/0304-3940(93)90005-6.
The effects of histamine (HA) receptor antagonists on hypoxia + hypoglycemia (ischemia)-induced impairment of 2-deoxyglucose (2-DG) uptake by rat hippocampal slices was evaluated. Hippocampal slices were exposed to 20-min ischemia and then returned to oxygenated and glucose-containing Krebs-Ringer solution for 6 h. Ischemia reduced 2-DG uptake in the hippocampal slices. The ischemia-induced reduction in 2-DG uptake was attenuated by pretreatment with H1 receptor antagonist but not with H2 receptor antagonist. Treatment with HA exacerbated the ischemia-induced decrease. The H1 receptor antagonist-induced neuroprotective effect was blocked by co-treatment with HA. The present study suggests that the blockade of H1 receptor-mediated function has a protective role in ischemia-induced decreases in glucose metabolism in hippocampal slices.
评估了组胺（HA）受体拮抗剂对缺氧+低血糖（缺血）诱导的大鼠海马切片2-脱氧葡萄糖（2-DG）摄取受损的影响。将海马切片暴露于20分钟的缺血状态，然后放回含氧量和葡萄糖的krebs-ringer溶液中6小时。缺血降低了海马切片中2-DG的摄取。用H1受体拮抗剂预处理可减轻缺血诱导的2-DG摄取减少，而用H2受体拮抗剂则不能。HA处理加剧了缺血诱导的减少。HA联合处理可阻断H1受体拮抗剂诱导的神经保护作用。本研究表明，阻断H1受体介导的功能对缺血诱导的海马切片葡萄糖代谢降低具有保护作用。
