Narváez J A, Aguirre J A, Fuxe K
Department of Physiology, University of Malaga, Spain.
Neurosci Lett. 1993 Mar 19;151(2):182-6. doi: 10.1016/0304-3940(93)90016-e.
The effects of NPY(13-36) on cardiorespiratory responses elicited by microinjections of L-glutamate into the nucleus tractus solitarius (NTS) have been studied in anaesthetized (alpha-chloralose + urethane) Sprague-Dawley rats. NPY(13-36) in doses ranging from 50 to 500 fmol produces a significant increase in mean arterial pressure (MAP) without significant effects on heart rate (HR) and respiratory rate (RR). The ED50 dose (100 fmol) of NPY(13-36) counteracts significantly the decrease in MAP and HR elicited by L-glutamate (1500 pmol) microinjected into NTS. Similar results are obtained using a threshold dose of the peptide (50 fmol) and an ED50 dose of L-glutamate (300 pmol). These results indicate that the Y2 receptors in NTS can mediate vasopressor responses to femtomolar amounts of NPY(13-36) and counteract cardiovascular responses to L-glutamate.
在麻醉（α-氯醛糖+乌拉坦）的斯普拉格-道利大鼠中，研究了神经肽Y（13 - 36）[NPY(13 - 36)]对向孤束核（NTS）微量注射L-谷氨酸所引发的心肺反应的影响。剂量范围为50至500飞摩尔的NPY(13 - 36)可使平均动脉压（MAP）显著升高，而对心率（HR）和呼吸频率（RR）无显著影响。NPY(13 - 36)的半数有效剂量（ED50，100飞摩尔）可显著对抗向NTS微量注射L-谷氨酸（1500皮摩尔）所引起的MAP和HR降低。使用该肽的阈剂量（50飞摩尔）和L-谷氨酸的ED50剂量（300皮摩尔）也得到了类似结果。这些结果表明，NTS中的Y2受体可介导对飞摩尔量NPY(13 - 36)的升压反应，并对抗对L-谷氨酸的心血管反应。
