Satoh K, Sasano H, Shimosegawa T, Koizumi M, Yamazaki T, Mochizuki F, Kobayashi N, Okano T, Toyota T, Sawai T
Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.
Cancer. 1993 Jul 1;72(1):51-6. doi: 10.1002/1097-0142(19930701)72:1<51::aid-cncr2820720112>3.0.co;2-o.
Intraductal mucin-hypersecreting neoplasm of the pancreas (IMHN) is a unique tumor that has a tendency to spread intraductally. The clinical outcome of IMHN generally is far better than that of pancreatic ductal cell carcinoma. Because of the presence of various cell atypia within the same tumor, it sometimes is difficult to make an accurate histopathologic diagnosis and, therefore, predict its biologic behavior. It has been shown that overexpression of c-erbB-2 protein in breast cancer with lymph node metastases is related to a poor prognosis. Overexpression of c-erbB-2 protein has been reported as an infrequent event in pancreatic ductal cell carcinoma, but little is known in the case of IMHN.
The expression of c-erbB-2 protein was immunohistochemically investigated in the formaldehyde-fixed, paraffin-embedded tissues of 17 cases of IMHN, and 14 cases of pancreatic ductal cell carcinoma (8 cases with lymph node metastasis), using polyclonal and monoclonal c-erbB-2(p185) antibodies by the avidin-biotin method.
Both the polyclonal and monoclonal antibodies showed similar immunostaining for the c-erbB-2 product. Overexpression of the c-erbB-2 product was observed frequently in IMHN (13/17), especially in that with moderate- to high-grade cell atypia (12/12), whereas it was detected in only 1 of 14 cases of pancreatic ductal cell carcinoma (1/14). Among eight cases of pancreatic ductal cell carcinoma with lymph node metastases, overexpression of the c-erbB-2 product in metastatic lesions was detected in two, one of whose primary lesions also overexpressed the oncogene product.
These observations suggest the genetic expression of c-erbB-2 is related to the pathogenesis of IMHN.
胰腺导管内黏液高分泌性肿瘤（IMHN）是一种独特的肿瘤，有沿导管内扩散的倾向。IMHN的临床结局通常远优于胰腺导管细胞癌。由于同一肿瘤内存在各种细胞异型性，有时难以做出准确的组织病理学诊断，因此也难以预测其生物学行为。研究表明，伴有淋巴结转移的乳腺癌中c-erbB-2蛋白过表达与预后不良有关。c-erbB-2蛋白过表达在胰腺导管细胞癌中报道较少，但在IMHN中的情况知之甚少。
采用抗生物素蛋白-生物素法，使用多克隆和单克隆c-erbB-2（p185）抗体，对17例IMHN及14例胰腺导管细胞癌（8例伴有淋巴结转移）的甲醛固定、石蜡包埋组织进行c-erbB-2蛋白表达的免疫组织化学研究。
多克隆和单克隆抗体对c-erbB-2产物显示出相似的免疫染色。c-erbB-2产物过表达在IMHN中常见（13/17），尤其是在中至高级别细胞异型性的病例中（12/12），而在14例胰腺导管细胞癌中仅1例检测到（1/14）。在8例伴有淋巴结转移的胰腺导管细胞癌中，2例转移灶检测到c-erbB-2产物过表达，其中1例原发灶也过表达该癌基因产物。
这些观察结果提示c-erbB-2的基因表达与IMHN的发病机制有关。
