Osako M, Yonezawa S, Siddiki B, Huang J, Ho J J, Kim Y S, Sato E
Second Department of Pathology, Kagoshima University School of Medicine, Japan.
Cancer. 1993 Apr 1;71(7):2191-9. doi: 10.1002/1097-0142(19930401)71:7<2191::aid-cncr2820710705>3.0.co;2-x.
Pancreatic cancer has a poor prognosis, and early diagnosis of carcinoma and discrimination between malignant and benign conditions are difficult. Many pancreatic cancer-associated antigens, such as CA 19-9, DU-PAN-2, YPan-1, and SPan-1, have been studied. However, expression of Tn, sialosyl-Tn, and T antigens in tissues of different types of pancreatic neoplasms has not been investigated systematically. Moreover, little is known about the distribution of different types of apomucins in the pancreas.
The expression of Tn, sialosyl-Tn, and T antigens and DF3 (mammary type apomucin) and intestinal MRP (intestinal type apomucin) was examined immunohistochemically in 47 pancreatic tumors: 36 invasive ductal carcinomas, 5 intraductal papillary tumors, and 6 adenomas.
In normal pancreatic tissues, neither Tn nor sialosyl-Tn antigen was expressed. In contrast, expression of both Tn and sialosyl-Tn antigens was observed in all the invasive ductal carcinomas and intraductal papillary tumors. None of the adenomas expressed both Tn and sialosyl-Tn. DF3 antigen was expressed in all invasive ductal carcinomas but not in intraductal papillary tumors, whereas intestinal MRP was expressed in all the intraductal papillary tumors but not in the invasive ductal carcinomas.
The results from this study suggest that the expression of the mucin core protein and mucin carbohydrate antigens is correlated with the biologic behavior of pancreatic tumors. In particular, the expression of mammary type mucin core protein and intestinal type mucin core protein showed a striking contrast between invasive ductal carcinomas with a poor prognosis and intraductal papillary tumors with a favorable prognosis.
胰腺癌预后较差，早期诊断癌症以及区分恶性和良性病变都很困难。许多与胰腺癌相关的抗原，如CA 19-9、DU-PAN-2、YPan-1和SPan-1，都已得到研究。然而，不同类型胰腺肿瘤组织中Tn、唾液酸Tn和T抗原的表达尚未得到系统研究。此外，关于不同类型脱辅基粘蛋白在胰腺中的分布也知之甚少。
采用免疫组织化学方法检测了47例胰腺肿瘤中Tn、唾液酸Tn和T抗原以及DF3（乳腺型脱辅基粘蛋白）和肠道MRP（肠型脱辅基粘蛋白）的表达，其中包括36例浸润性导管癌、5例导管内乳头状肿瘤和6例腺瘤。
在正常胰腺组织中，Tn和唾液酸Tn抗原均未表达。相反，在所有浸润性导管癌和导管内乳头状肿瘤中均观察到Tn和唾液酸Tn抗原的表达。所有腺瘤均未同时表达Tn和唾液酸Tn。DF3抗原在所有浸润性导管癌中表达，但在导管内乳头状肿瘤中不表达，而肠道MRP在所有导管内乳头状肿瘤中表达，但在浸润性导管癌中不表达。
本研究结果表明，粘蛋白核心蛋白和粘蛋白碳水化合物抗原的表达与胰腺肿瘤的生物学行为相关。特别是，乳腺型粘蛋白核心蛋白和肠型粘蛋白核心蛋白的表达在预后较差的浸润性导管癌和预后较好的导管内乳头状肿瘤之间形成了鲜明对比。
