Harvey D, Harrington C, Heuzenroeder M W, Murray C
Division of Clinical Microbiology, Institute of Medical and Veterinary Science, Adelaide, Australia.
FEMS Microbiol Lett. 1993 Apr 15;108(3):291-5. doi: 10.1111/j.1574-6968.1993.tb06118.x.
A phage typing system using a group of 11 closely related phage (as judged by Southern analysis and restriction fragment length polymorphism analysis) was able to distinguish at least six phage types in Salmonella heidelberg of human and animal origin. Restriction fragment length polymorphism analysis using cosmid probes from S. heidelberg confirmed that most S. heidelberg isolates belong to a single 'clonal' group. Southern analysis using DNA isolated from each of the testing phage group showed that phage types 4, 5 and 6 carry closely related endogenous or lysogenic phage. Induction of a lysogenic phage Hlp-4 (Heidelberg lysogenic phage) from type 4 could become lysogenic and convert phage types 1 and 3 to phage type 4 and phage type 5 to a non-typable phenotype.
一个噬菌体分型系统使用一组11种密切相关的噬菌体（通过Southern分析和限制性片段长度多态性分析判断），能够区分来源于人和动物的海德堡沙门氏菌中的至少六种噬菌体类型。使用来自海德堡沙门氏菌的黏粒探针进行的限制性片段长度多态性分析证实，大多数海德堡沙门氏菌分离株属于单一的“克隆”群体。使用从每个测试噬菌体组分离的DNA进行的Southern分析表明，噬菌体类型4、5和6携带密切相关的内源或溶原性噬菌体。从类型4诱导出溶原性噬菌体Hlp-4（海德堡溶原性噬菌体）可变为溶原性，并将噬菌体类型1和3转变为噬菌体类型4，将噬菌体类型5转变为不可分型的表型。
