Cance W G, Craven R J, Weiner T M, Liu E T
Department of Surgery, Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill 27599.
Int J Cancer. 1993 Jun 19;54(4):571-7. doi: 10.1002/ijc.2910540409.
The family of protein kinases includes many oncogenes and growth-factor receptors, as well as genes that are involved in cell-cycle regulation. We have identified protein kinases expressed in a human breast-cancer cell line, 600PEI, and a primary human breast carcinoma, using PCR cloning techniques based on consensus sequences in the kinase domain. Twenty-five different protein kinases were isolated, including 3 novel putative tyrosine kinases (designated TK1, TK2, and TK5), and 2 novel putative cell-cycle-associated serine/threonine kinases (designated STK1 and STK2). TK1 is a new member of the src family of kinases that is expressed predominantly in epithelial cells. TK2 is homologous to the receptor kinase, HEK, and TK5 appears to be another member of the JAK family of kinases. The novel serine/threonine kinases, designated STK1 and STK2, were homologous to the human cdc2 and the Aspergillus nimA genes. We subsequently analyzed the levels of expression of all of these protein kinases in a panel of human breast carcinomas, using PCR-based methods. This analysis revealed different expression profiles in different primary breast carcinomas and, therefore, may determine new molecular sub-sets of human breast cancer.
蛋白激酶家族包括许多癌基因和生长因子受体，以及参与细胞周期调控的基因。我们利用基于激酶结构域共有序列的PCR克隆技术，在人乳腺癌细胞系600PEI和原发性人乳腺癌中鉴定出了表达的蛋白激酶。分离出了25种不同的蛋白激酶，包括3种新的假定酪氨酸激酶（命名为TK1、TK2和TK5），以及2种新的假定细胞周期相关丝氨酸/苏氨酸激酶（命名为STK1和STK2）。TK1是src激酶家族的新成员，主要在上皮细胞中表达。TK2与受体激酶HEK同源，TK5似乎是JAK激酶家族的另一个成员。命名为STK1和STK2的新型丝氨酸/苏氨酸激酶与人cdc2和曲霉nimA基因同源。随后，我们使用基于PCR的方法分析了一组人乳腺癌中所有这些蛋白激酶的表达水平。该分析揭示了不同原发性乳腺癌中的不同表达谱，因此可能确定人乳腺癌的新分子亚组。
