Rodriguez-Peralta L
Am J Ophthalmol. 1975 Oct;80(4):713-25. doi: 10.1016/0002-9394(75)90405-5.
The in vivo sites of the blood-aqueous barrier were determined in five animal species, using acriflavine neutral (224 mol. wt.), ultrafreezing and drying, fluorescence microscopy, and fluorometry. Not toxic, acriflavine had specific in vivo affinity for nuclear DNA and RNA, produced intense fluorescence, and did not pass through hematic barriers. Acriflavine was given in doses to produce the same concentrations in the systemic blood or in the aqueous humor. The exact sites of the blood-aqueous barrier, demonstrated by tracing the acriflavine fluorescence through tissues and individual cells, were in the basal cell membranes of inner ciliary and iridial epithelia and apical cell membranes of iridial and corneal endothelia. Acriflavine passed freely from the aqueous humor into the blood stream. It also followed an aqueous flow into the vitreous body and optic nerve head vessels. Interruption or reversal of this flow may cause open-angle glaucoma.
利用中性吖啶黄（分子量224）、超速冷冻干燥、荧光显微镜检查和荧光测定法，在五种动物物种中确定了血-房水屏障的体内部位。吖啶黄无毒，对核DNA和RNA具有特定的体内亲和力，产生强烈荧光，且不穿过血屏障。给予吖啶黄一定剂量，使其在全身血液或房水中产生相同浓度。通过追踪组织和单个细胞中的吖啶黄荧光来证明，血-房水屏障的确切部位在内睫状上皮和虹膜上皮的基底细胞膜以及虹膜和角膜内皮的顶端细胞膜。吖啶黄可从房水自由进入血流。它还随房水流入玻璃体和视神经乳头血管。这种流动的中断或逆转可能导致开角型青光眼。
