Vossen J M
Ann N Y Acad Sci. 1975 Jun 30;254:262-79. doi: 10.1111/j.1749-6632.1975.tb29176.x.
Lymphocytes from bone marrow and blood of infants and children and from various tissues of seven fetuses between 13 and 25 weeks of gestational age were studied by immunofluorescence for the presence of membrane-associated immunoglobulins. Cells with mu and delta determinants prevailed, and these immunoglobulins were usually present on the same cell. The bone marrow specimens of all age groups contained more mu-positive cells without delta determinants than did the samples from the peripheral blood. Such mu(+)delta(-) cells were also found in tissues of young fetuses. These cells were postulated to represent the first stage of B cells displaying Ig receptors. Other tests with double staining showed that cells with delta determinants, but without the presence of other H chains, are rare, if they exist at all. This finding supports the idea that the role of IgD could be one of regulation of antibody secretion rather than of antibody activity. The number of cells that displayed alpha determinants was first observed to rise after birth, and only in the bone marrow, where it reached an average maximum of 16%. The relative number of such cells in the blood never exceeded a few percent.
通过免疫荧光法研究了婴儿和儿童骨髓及血液中的淋巴细胞，以及孕龄在13至25周之间的7个胎儿不同组织中的淋巴细胞，以检测膜相关免疫球蛋白的存在情况。带有μ和δ决定簇的细胞占主导，且这些免疫球蛋白通常存在于同一细胞上。所有年龄组的骨髓标本中，不含δ决定簇的μ阳性细胞比外周血标本中的更多。在年轻胎儿的组织中也发现了此类μ(+)δ(-)细胞。推测这些细胞代表了显示Ig受体的B细胞的第一阶段。其他双重染色试验表明，带有δ决定簇但不存在其他重链的细胞即便存在也很罕见。这一发现支持了IgD的作用可能是调节抗体分泌而非抗体活性的观点。显示α决定簇的细胞数量首次被观察到在出生后增加，且仅在骨髓中增加，在骨髓中平均最高可达16%。此类细胞在血液中的相对数量从未超过百分之几。
