Grund S, Seiler A
Institut für Mikrobiologie und Tierhygiene des Fachbereichs Veterinärmedizin, Freien Universität Berlin, Deutschland.
Zentralbl Veterinarmed B. 1993 Mar;40(2):105-12.
On Salmonella typhimurium variatio copenhagen (STMVC) strains, isolated from pigeons with acute Salmonellosis, two types of fimbriae can be identified, depending on different growth conditions. In addition to the common 7 nm fimbriae we were able to demonstrate in the electron microscope, thin, curled fimbriae 3 nm in diameter which are characterized by a mannose-resistant hemagglutination. The simultaneous expression of both types of fimbriae on a single bacterial cell can be induced by transferring a microcolony from agar medium (3 nm fimbriae), to the surface of a nutrient broth (7 nm fimbriae) and continuing the incubation. One selected strain expresses only the thin fimbriae on nearly all the bacteria on an agar medium. These thin fimbriae seem to play a role in the lectinophagocytosis in macrophage cultures. The attachment of Salmonellae mediated by these fimbriae as well as the internalization of fimbriated cells by macrophages is shown in the electron microscope and discussed in respect to infection and immunization.
从患有急性沙门氏菌病的鸽子中分离出的鼠伤寒沙门氏菌哥本哈根变种（STMVC）菌株，根据不同的生长条件可鉴定出两种菌毛。除了我们能够在电子显微镜下观察到的常见的7纳米菌毛外，还有直径为3纳米的纤细卷曲菌毛，其特征是具有抗甘露糖血凝反应。通过将微菌落从琼脂培养基（3纳米菌毛）转移到营养肉汤表面（7纳米菌毛）并继续培养，可以诱导单个细菌细胞同时表达这两种菌毛。选择的一个菌株在琼脂培养基上几乎所有细菌上仅表达纤细菌毛。这些纤细菌毛似乎在巨噬细胞培养中的凝集吞噬作用中发挥作用。通过这些菌毛介导的沙门氏菌附着以及有菌毛细胞被巨噬细胞内化的过程在电子显微镜下可见，并就感染和免疫进行了讨论。
