Craven S E, Williams D D
Poultry Microbiological Safety Research Unit, USDA, Richard B. Russell Agricultural Research Center, Athens, Georgia 30604-5677, USA.
Avian Dis. 1997 Jul-Sep;41(3):548-58.
The ability of selected strains of Enterobacteriaceae or lactobacilli isolated from the intestines of adult chickens to inhibit in vitro attachment of Salmonella typhimurium 3333/O to cecal mucus in the presence or absence of D-mannose was determined. Attachment in the absence of mannose was reduced by prior exposure of mucus to cultures of two isolates of Enterobacteriaceae, an Escherichia coli and a Hafnia alvei strain, but not to a third isolate, an Enterobacter agglomerans strain. Attachment of S. typhimurium was not inhibited when mannose was present in the blocking or attachment step. Formation of fimbriae by the two inhibitory Enterobacteriaceae strains and the S. typhimurium strain, as indicated by titers of mannose-sensitive hemagglutination of guinea pig erythrocytes was optimal in Z biphasic medium (consisting of tryptone, yeast extract, dextrose, and NaCl) incubated anaerobically at 42 C. Fimbriae of each of three strains prepared from these cultures also inhibited attachment. These are characteristics consistent with attachment and inhibition of attachment mediated by a mannose-sensitive adhesin associated with type 1 fimbriae on bacterial cells of Enterobacteriaceae strains. Attachment in the presence of mannose was significantly reduced by prior exposure of mucus to cultures of a Lactobacillus salivarius strain and a Lactobacillus delbrueckii delbrueckii strain but not to a strain of Lactobacillus for which the species had not been determined. Washed cells or spent culture supernatant fluid from brain-heart infusion broth, Z broth, or Z biphasic cultures of the inhibitory strains of lactobacilli incubated at 37 or 42 C inhibited this form of attachment. Of 27 intestinal isolates of Enterobacteriaceae and 21 of lactobacilli, the lactobacilli strains were generally more hydrophobic than the Enterobacteriaceae as determined by adherence to hexadecane. The lactobacilli isolates did not agglutinate guinea pig erythrocytes. The data suggest more than one mechanism for mediating attachment of inhibitory bacterial strains and for subsequent attachment of S. typhimurium.
测定了从成年鸡肠道分离出的某些肠杆菌科菌株或乳酸杆菌菌株，在有或没有D - 甘露糖存在的情况下，体外抑制鼠伤寒沙门氏菌3333/O附着于盲肠黏液的能力。在没有甘露糖的情况下，将黏液预先暴露于两种肠杆菌科分离株（一种大肠杆菌和一种蜂房哈夫尼亚菌菌株）的培养物中，鼠伤寒沙门氏菌的附着减少，但暴露于第三种分离株（成团肠杆菌菌株）时则没有减少。当在封闭或附着步骤中存在甘露糖时，鼠伤寒沙门氏菌的附着未受到抑制。两种具有抑制作用的肠杆菌科菌株和鼠伤寒沙门氏菌菌株形成菌毛的情况，通过豚鼠红细胞对甘露糖敏感的血凝滴度表明，在42℃厌氧培养的Z双相培养基（由胰蛋白胨、酵母提取物、葡萄糖和氯化钠组成）中最佳。从这些培养物中制备的三种菌株的菌毛也抑制了附着。这些特征与由与肠杆菌科菌株细菌细胞上1型菌毛相关的甘露糖敏感黏附素介导的附着和附着抑制一致。在有甘露糖存在的情况下，将黏液预先暴露于唾液乳杆菌菌株和德氏乳杆菌保加利亚亚种菌株的培养物中，附着显著减少，但暴露于一种未确定种属的乳杆菌菌株时则没有减少。在37℃或42℃培养的抑制性乳酸杆菌菌株的脑心浸液肉汤、Z肉汤或Z双相培养物中的洗涤细胞或用过的培养上清液抑制了这种附着形式。在27株肠杆菌科肠道分离株和21株乳酸杆菌中，通过对十六烷的黏附测定，乳酸杆菌菌株通常比肠杆菌科菌株更疏水。乳酸杆菌分离株不凝集豚鼠红细胞。数据表明，介导抑制性细菌菌株附着以及随后鼠伤寒沙门氏菌附着的机制不止一种。
