Ueda A, Ito M, Ishiguro N, Miura Y, Asai J, Miura T
Department of Orthopaedic Surgery, Nagoya University School of Medicine, Japan.
Transplantation. 1993 Jul;56(1):124-7. doi: 10.1097/00007890-199307000-00023.
Dendritic-shaped cells (Thy-1+DC) characterized by the surface phenotype of Thy-1+, asialo GM1+, Ia- are reported to exist exclusively in mice. However, Thy-1+ dendritic cells were observed in the skin of normal athymic nude F344, euthymic F344, and WKAH rats. Their phenotypic markers were Thy-1+, asialo GM1+, Ia-, W3/25 (helper/inducer)-, OX8 (cytotoxic/suppressor)-, W3/13 (pan T lymphocytes)-, OX39 (IL-2)-, and OX41 (macrophages)-. These phenotypic markers were analogous to those of mice. The density of Thy-1+DC in the skin of normal nude F344 rats was approximately twice as much as that of F344 and WKAH rats. When WKAH rats were used as donors with nude F344 rats as recipients (this combination being a major mismatch with regard to a MHC, rejection-like changes such as edema and erythema were observed 1 week after the grafting. An immunohistochemical examination demonstrated that the density of Thy-1+DC had increased 3-fold within the donor skin at 1 week after transplantation, although almost no W3/25 positive cells, OX8 positive cells, or other inflammatory cells were observed. Within 2 weeks of transplantation the donor skin ceased rejection-like changes and the density of Thy-1+DC was reduced to preoperation levels. When WKAH rats were used as donors with F344 rats as recipients, severe rejection was observed, and many T cells with W3/25 or OX8 antigens and granulocytes had infiltrated into the skin at the donor sites. Thy-1+DC could not be found in the donor skin. These findings suggest that a correlation exists between Thy-1+DC and rejection-like changes in the donor skin when nude rats are used as recipients.
据报道，具有Thy-1+、无唾液酸GM1+、Ia-表面表型的树突状细胞（Thy-1+DC）仅存在于小鼠中。然而，在正常无胸腺裸F344大鼠、有胸腺的F344大鼠和WKAH大鼠的皮肤中观察到了Thy-1+树突状细胞。它们的表型标志物为Thy-1+、无唾液酸GM1+、Ia-、W3/25（辅助/诱导）-、OX8（细胞毒性/抑制）-、W3/13（全T淋巴细胞）-、OX39（白细胞介素-2）-和OX41（巨噬细胞）-。这些表型标志物与小鼠的类似。正常裸F344大鼠皮肤中Thy-1+DC的密度约为F344大鼠和WKAH大鼠的两倍。当以WKAH大鼠为供体、裸F344大鼠为受体时（这种组合在MHC方面存在主要错配），移植后1周观察到移植部位出现水肿和红斑等类似排斥的变化。免疫组织化学检查表明，移植后1周，供体皮肤内Thy-1+DC的密度增加了3倍，尽管几乎未观察到W3/25阳性细胞、OX8阳性细胞或其他炎症细胞。移植后2周内，供体皮肤停止出现类似排斥的变化，Thy-1+DC的密度降至术前水平。当以WKAH大鼠为供体、F344大鼠为受体时，观察到严重排斥反应，许多带有W3/25或OX8抗原的T细胞和粒细胞浸润到供体部位的皮肤中。在供体皮肤中未发现Thy-1+DC。这些发现表明，当以裸鼠为受体时，Thy-1+DC与供体皮肤中类似排斥的变化之间存在相关性。
