Conner R L, Reilly A E
Biochim Biophys Acta. 1975 Aug 25;398(2):209-16. doi: 10.1016/0005-2760(75)90136-8.
Cultures of Tetrahymena pyriformis W respond to isovalerate supplementation by an increase in odd-numbered saturated, unsaturated and alpha-hydroxy iso-fatty acids and by a decrease in even-numbered normal fatty acids in the glycerolipids and sphingolipids. Supplementation, however, did not alter the relative amount of unsaturated fatty acids found in the polar lipids. The unsaturated acids 17 : 1(i) and 19 : 1(i) were isolated from cells grown with [1-14C]isovalerate and found to have a higher specific activity than the monoenes of the normal series. Isotopic and gas-chromatographic analyses also indicated the presence of dienoic and trienoic acids of the iso-acid series. The iso-fatty acid content was elevated with isovalerate levels up to 5.0 mM and an inhibition of growth was noted. At higher concentrations of the short chain precursor, no further increase in total cellular iso-acids was detected although growth inhibition was more pronounced. The alpha-hydroxy iso-fatty acids of the sphingolipids, however, were elevated in a fashion that paralleled the external concentration of isovalerate; thus, the amount of alpha-hydroxy iso-acids and the degree of growth inhibition show a direct relationship. The increase in alpha-hydroxy iso-acid content of the sphingolipids was at the expense of the saturated normal and iso-acid components. The impact on the physiology of the cells can be envisaged as the result of changes in membrane fluidity due to the presence of high levels of iso-fatty acids without an accompanying reduction in unsaturated acids.
