Frisch A, Loyter A, Levy R, Goldberg I
Biochim Biophys Acta. 1978 Jan 4;506(1):18-29. doi: 10.1016/0005-2736(78)90431-5.
Conjugation in Tetrahymena pyriformis is induced by the mixing of two starved complementary mating types. Addition of the antibiotic cerulenin, a specific inhibitor of de novo lipid synthesis, upon mixing of the mating types inhibited the conjugation process. The inhibition of conjugation was found to be reversible upon washing the cells. Cerulenin inhibited [14C]acetate incorporation into the lipid fraction of the cells, while it did not affect the incorporation of [3H]leucine into proteins. Analysis of the fatty acid composition of the whole cells revealed that during conjugation the ratio of saturated to unsaturated fatty acids is markedly changed. While the ratio of saturated:unsaturated fatty acids is 0.30 in unconjugated cells, it reached a value of 0.45 in conjugated cells.
梨形四膜虫的接合作用是由两种饥饿的互补交配型混合诱导产生的。在交配型混合时添加抗生素浅蓝菌素（一种从头合成脂质的特异性抑制剂）会抑制接合过程。发现洗涤细胞后，接合作用的抑制是可逆的。浅蓝菌素抑制[14C]乙酸掺入细胞的脂质部分，而不影响[3H]亮氨酸掺入蛋白质。对全细胞脂肪酸组成的分析表明，在接合过程中饱和脂肪酸与不饱和脂肪酸的比例发生了显著变化。未接合细胞中饱和脂肪酸:不饱和脂肪酸的比例为0.30，而在接合细胞中该比例达到了0.45。
