Liu C M, Hou L T
Department of Dentistry, National Taiwan University Hospital, Taipei, R.O.C.
J Formos Med Assoc. 1993 Feb;92(2):157-64.
A total of 51 gingival crevicular fluid (GCF) samples were collected, and their associated gingival index (GI) and probing pocket depth (PD) were recorded. The active and latent collagenase activities in the GCF were determined by an assay measuring the intact alpha 1 chain of collagen after sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and autoradiography with 3H-collagen (type I) as an enzyme substrate. The collagen digestion patterns showed that GCF samples generated predominantly 3/4 alpha and 1/4 alpha collagen fragments, typical of vertebrate collagenase. The active collagenase activity was significantly associated with the severity of the measured GI. Active enzyme activity was also elevated in sites with attachment loss (PD > 4mm). However, the enzyme activity in the group with a 7-10 mm probing depth was not greater than that of those with a 4-6 mm pocket depth. The elevation of active collagenase activity was found to be highly correlated with GI, while it was low with respect to the probing depth. The latent collagenase activity increased with the GI score, but not with the pocket depth. These results suggest that most collagenase present in GCF is derived from polymorphonuclear leukocytes which are markedly increased in more severe cases of periodontal disease. Thus, the measurement of active collagenase in GCF samples of patients is a scientifically reliable and sensitive method of assessing disease activity possibly associated with tissue destruction.
共收集了51份龈沟液（GCF）样本，并记录了其相关的牙龈指数（GI）和探诊深度（PD）。通过一种测定方法来确定GCF中的活性和潜在胶原酶活性，该方法是在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）后，以3H-胶原蛋白（I型）作为酶底物进行放射自显影，测量完整的α1胶原蛋白链。胶原蛋白消化模式显示，GCF样本主要产生3/4α和1/4α胶原蛋白片段，这是脊椎动物胶原酶的典型特征。活性胶原酶活性与所测GI的严重程度显著相关。在有附着丧失（PD>4mm）的部位，活性酶活性也升高。然而，探诊深度为7 - 10mm组的酶活性并不高于探诊深度为4 - 6mm组。发现活性胶原酶活性的升高与GI高度相关，而与探诊深度相关性较低。潜在胶原酶活性随GI评分增加，但不随探诊深度增加。这些结果表明，GCF中存在的大多数胶原酶来源于多形核白细胞，在更严重的牙周病病例中其数量明显增加。因此，测量患者GCF样本中的活性胶原酶是评估可能与组织破坏相关的疾病活动的一种科学可靠且敏感的方法。
