Safkan-Seppälä Bedia, Sorsa Timo, Tervahartiala Taina, Beklen Arzu, Konttinen Yrjö T
Institute of Dentistry, University of Helsinki, and Department of Medicine/Invärtes Medicin, Helsinki University Hospital, Helsinki, Finland.
J Periodontol. 2006 Feb;77(2):189-94. doi: 10.1902/jop.2006.040322.
Studies have demonstrated that high levels of collagenase activity in gingival crevicular fluid (GCF) are associated with degradation of periodontal tissues in progressive periodontitis compared to periodontally healthy tissues. Because the activation of collagenases is an important issue in periodontitis, we have studied the activation of collagenase in gingival crevicular fluid samples of diabetic patients.
Collagenase activity was studied in human gingival crevicular fluids. Twenty-two poorly controlled diabetic patients (e.g., blood glucose: 11.0+/-0.7 mmol/l; hemoglobin A1c [HbA1c]: 9.6%+/-0.3%) and five well-controlled diabetic patients were compared to six chronic periodontitis subjects and five healthy controls. Collagenase activity against type I collagen was measured using sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis quantitated by laser densitometry.
The poorly controlled diabetic patients had more alveolar bone loss than the well-controlled diabetic subjects and controls (P<0.001; t test). The activity of collagenases in GCF in poorly controlled diabetic patients was similar to that seen in chronic periodontitis subjects (P>0.05) but higher than in healthy controls (P<0.01; t test), whereas there was no difference between the well-controlled diabetic subjects and systemically healthy controls (P>0.05; t test).
Poorly controlled diabetes is strongly related to periodontal tissue destruction, and collagenases in GCF may mediate and reflect this effect.
研究表明，与牙周健康组织相比，进展期牙周炎患者龈沟液（GCF）中高水平的胶原酶活性与牙周组织的破坏有关。由于胶原酶的激活是牙周炎中的一个重要问题，我们研究了糖尿病患者龈沟液样本中胶原酶的激活情况。
对人类龈沟液中的胶原酶活性进行了研究。将22名血糖控制不佳的糖尿病患者（例如，血糖：11.0±0.7 mmol/l；糖化血红蛋白[HbA1c]：9.6%±0.3%）和5名血糖控制良好的糖尿病患者与6名慢性牙周炎患者和5名健康对照进行比较。使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析并通过激光密度测定法定量，测量针对I型胶原的胶原酶活性。
血糖控制不佳的糖尿病患者比血糖控制良好的糖尿病患者和对照组有更多的牙槽骨丧失（P<0.001；t检验）。血糖控制不佳的糖尿病患者龈沟液中胶原酶的活性与慢性牙周炎患者相似（P>0.05），但高于健康对照组（P<0.01；t检验），而血糖控制良好的糖尿病患者与全身健康对照组之间没有差异（P>0.05；t检验）。
血糖控制不佳与牙周组织破坏密切相关，龈沟液中的胶原酶可能介导并反映这种作用。
