牛精囊前列腺素合成酶的酶学性质

Enzymatic properties of prostaglandin synthetase from bovine seminal vesicles.

作者信息

Duvivier J, Wolf D, Heusghem C

出版信息

Biochimie. 1975;57(5):521-8. doi: 10.1016/s0300-9084(75)80131-3.

Abstract

The relative quantities of PGE2 and PGF2alpha biosynthesized are strictly dependent on the concentration of the precursor, arachidonate, on the nature and on the quantity of sulfhydrylated cofactor present. The sigmoid aspect of the curve of PGF2alpha biosynthesis as a function of arachidonate concentration is directly dependent on the GSH concentration. Treatment of the microsomes by low concentrations of HgCl (5 muM) modifies the kinetics of PGE2 and PGF2alpha biosynthesis. Similarly, the presence of L-8027, a competitive inhibitor, alters qualitatively and quantitatively the production of PGE2 and PGF2alpha as a function of arachidonate concentration.

摘要

生物合成的前列腺素E2（PGE2）和前列腺素F2α（PGF2α）的相对量严格取决于前体花生四烯酸的浓度、存在的巯基化辅因子的性质和数量。PGF2α生物合成曲线作为花生四烯酸浓度的函数的S形与谷胱甘肽（GSH）浓度直接相关。用低浓度的HgCl（5 μM）处理微粒体会改变PGE2和PGF2α生物合成的动力学。同样，竞争性抑制剂L-8027的存在会定性和定量地改变PGE2和PGF2α作为花生四烯酸浓度函数的产生。