Bontempi E J, Búa J, Aslund L, Porcel B, Segura E L, Henriksson J, Orn A, Pettersson U, Ruiz A M
Instituto Nacional de Diagnóstico e Investigación de la Enfermedad de Chagas, Ministerio de Salud y Acción Social, Buenos Aires, Argentina.
Mol Biochem Parasitol. 1993 Jun;59(2):253-62. doi: 10.1016/0166-6851(93)90223-k.
The complete sequence of a gene encoding a 46-kDa protein of Trypanosoma cruzi is presented. The first ATG complies with the consensus sequence for initiation of translation. A single band of 2 kb was highlighted by hybridizing a probe from the 46-kDa protein gene to a Northern filter containing total T. cruzi RNA. The gene is present in 50-80 copies per cell and most of them are contained in 2 tandem arrays on large T. cruzi chromosomes (> 2000 kb). A strong homology with rat and human tyrosine aminotransferase was detected. Homology with a Trypanosoma brucei retrotransposon was found in the nonsense strand of the intergenic region.
本文展示了编码克氏锥虫46 kDa蛋白的基因的完整序列。首个ATG符合翻译起始的共有序列。通过将来自46 kDa蛋白基因的探针与含有克氏锥虫总RNA的Northern印迹杂交，突出显示了一条2 kb的单带。该基因在每个细胞中以50 - 80个拷贝存在，其中大部分包含在克氏锥虫大染色体（> 2000 kb）上的2个串联阵列中。检测到与大鼠和人酪氨酸转氨酶有很强的同源性。在基因间隔区的无义链中发现了与布氏锥虫反转录转座子的同源性。
