Paz Aliaga B, Troyano Pueyo M, Noriega Ponce P, Lara F J
Cell Differ. 1975 Oct;4(4):243-55. doi: 10.1016/0045-6039(75)90030-5.
Polyribosomes were obtained in high yield from Rhynchosciara salivary glands by a method which uses the non-ionic detergent Nonidet P 40, in moderate salt buffer and DTT as RNase inhibitor. The polysomal profiles from glands of animals which have just started to spin the communal cocoon are slightly but consistently different from those obtained from about 5 day older animals, in which DNA puffs were open in the salivary gland chromosomes. The heaviest polyribosomes which could be detected by either radioactive uridine or amino acid incorporation have 8 to 9 ribosomes. The data indicate the existence of stable messengers in the salivary glands.
通过一种使用非离子去污剂Nonidet P 40、中等盐缓冲液和作为核糖核酸酶抑制剂的二硫苏糖醇（DTT）的方法，从南美大蠊唾液腺中高产率地获得了多核糖体。刚开始吐公共茧的动物腺体的多核糖体图谱与约5天大的动物的图谱略有但持续存在差异，在约5天大的动物中，唾液腺染色体中的DNA泡是开放的。通过放射性尿苷或氨基酸掺入检测到的最重的多核糖体有8到9个核糖体。数据表明唾液腺中存在稳定的信使。
