Polysomal RNA in Semliki Forest virus infected Aedes albopictus cells.

Polysomes from Aedes albopictus cells were identified by their rapid labelling with radioactive amino acids and their sensitivity to EDTA, RNase and puromycin. The major ribosome component in cytoplasmic extracts had a sedimentation coefficient of approx. 95S and may be a ribosome dimer. In Semiki Forest virus infected Aedes albopictus cells, 42S and 26S RNA were the major virus RNA species detected up to 10 h post infection. Virus RNA was detected in association with pre-labelled ribosomes and banded at a buoyant density of 1-55 g/ml. 42S, 38S and 26S virus RNA species were associated with polysomes.