采用高效液相色谱-荧光检测法测定血浆中新型麦角灵衍生物FCE 23884及其可能的代谢产物6-去甲衍生物。

Determination of FCE 23884, a new ergolene derivative, and its possible metabolite, the 6-nor-derivative in plasma by high-performance liquid chromatography with fluorescence detection.

作者信息

Marrari P, Basileo G, Pianezzola E, Strolin Benedetti M

机构信息

Farmitalia Carlo Erba, Research and Development, Erbamont Group, Milan, Italy.

出版信息

J Pharm Biomed Anal. 1993 Apr-May;11(4-5):393-9. doi: 10.1016/0731-7085(93)80035-y.

DOI:10.1016/0731-7085(93)80035-y

PMID:8102884

Abstract

A sensitive and selective high-performance liquid chromatographic method for the determination of FCE 23884 and its 6-nor-derivative (FCE 26506) in plasma has been developed. After buffering the plasma samples, the compounds and the internal standard were extracted with ethyl ether-n-octanol (9:1, v/v), back-extracted into 0.01 M phosphoric acid and then analysed by reversed-phase liquid chromatography. Quantification was achieved by fluorescence detection of the eluate. The linearity, precision and accuracy of the method were evaluated. No interference from the biological matrix was observed. The assay was adequate for the quantification of plasma levels of the two compounds after a single oral dose of 1 mg of FCE 23884 in humans.

摘要

已开发出一种灵敏且具选择性的高效液相色谱法，用于测定血浆中FCE 23884及其6-去甲衍生物（FCE 26506）。将血浆样品缓冲后，用乙醚-正辛醇（9:1，v/v）萃取化合物及内标，再反萃取至0.01 M磷酸中，然后通过反相液相色谱进行分析。通过对洗脱液进行荧光检测实现定量。评估了该方法的线性、精密度和准确度。未观察到生物基质的干扰。该测定法适用于在人体单次口服1 mg FCE 23884后对两种化合物的血浆水平进行定量。