来自猪（家猪）胸腺的肌苷单磷酸脱氢酶：纯化及性质

Inosine monophosphate dehydrogenase from porcine (Sus scrofa domestica) thymus: purification and properties.

Pugh M E, Skibo E B

Department of Chemistry, Bloomsburg University, PA 17815.

Comp Biochem Physiol B. 1993 Jun;105(2):381-7. doi: 10.1016/0305-0491(93)90245-z.

Abstract

IMP dehydrogenase (EC 1.1.1.205) from porcine thymus glands has been purified to homogeneity. 2. The enzyme has a subunit MW of 57 kDa and an amino acid composition similar to those obtained from other normal and cancerous mammalian cells. 3. The apparent Km values at pH 8.0 for IMP and NAD+ are 7 and 16 microM, respectively. 4. GMP, XMP and AMP are competitive inhibitors towards IMP and Ki values of 50, 85 and 282 microM, respectively. 5. The effectiveness of nucleotides to protect inactivation by CI-IMP is IMP > GMP > XMP > AMP.

摘要

已将来自猪胸腺的肌苷酸脱氢酶（EC 1.1.1.205）纯化至同质。2. 该酶的亚基分子量为57 kDa，其氨基酸组成与从其他正常和癌性哺乳动物细胞中获得的相似。3. 在pH 8.0时，IMP和NAD+的表观Km值分别为7和16 microM。4. GMP、XMP和AMP是IMP的竞争性抑制剂，Ki值分别为50、85和282 microM。5. 核苷酸对CI-IMP保护失活的有效性为IMP > GMP > XMP > AMP。