Slierendregt B L, Otting N, Jonker M, Bontrop R E
Department of Chronic and Infectious diseases, Institute for Applied Radiobiology and Immunology TNO, Rijswijk, The Netherlands.
Tissue Antigens. 1993 Apr;41(4):178-85. doi: 10.1111/j.1399-0039.1993.tb01999.x.
By the use of restriction fragment length polymorphism analysis 10 Taq I fragments could be identified for the MhcMamu-DQA1 region. A strong correlation exists between the occurrence of Mamu-DQA1/Taq I fragments and Mamu-DQA1 allelic sequence variation. Most restriction fragments correspond with a unique Mamu-DQA1 allele, with one exception being the Taq I 4.5 kb fragment that is associated with two Mamu-DQA1 alleles. The RFLP technique allowed the identification of 15 Mamu-DQB1/Taq I restriction fragments, whereas sequence analysis has permitted the characterization of at least 20 different Mamu-DQB1 alleles. In this communication two unpublished Mamu-DQB1 sequences are described. For Mamu-DQB1, on only four occasions was it possible to demonstrate a correlation between a certain fragment and an allelic sequence. These analyses, performed on material from truly homozygous animals, allowed us to define which combinations of Mamu-DQA1 and -DQB1 molecules form heterodimers at the cell surface. In addition, these studies are helpful in typing non-human primate species that are used in biomedical research.
通过限制性片段长度多态性分析，可鉴定出MhcMamu - DQA1区域的10个Taq I片段。Mamu - DQA1/Taq I片段的出现与Mamu - DQA1等位基因序列变异之间存在很强的相关性。大多数限制性片段对应一个独特的Mamu - DQA1等位基因，唯一的例外是4.5 kb的Taq I片段，它与两个Mamu - DQA1等位基因相关。RFLP技术可鉴定出15个Mamu - DQB1/Taq I限制性片段，而序列分析已鉴定出至少20种不同的Mamu - DQB1等位基因。在本通讯中描述了两个未发表的Mamu - DQB1序列。对于Mamu - DQB1，仅在四次实验中有可能证明某个片段与等位基因序列之间存在相关性。对来自真正纯合动物的材料进行的这些分析，使我们能够确定Mamu - DQA1和 - DQB1分子的哪些组合在细胞表面形成异二聚体。此外，这些研究有助于对用于生物医学研究的非人类灵长类物种进行分型。
