恒河猴的DQ单倍型分析：对这些基因进化的启示
DQ-haplotype analysis in rhesus macaques: implications for the evolution of these genes.
作者信息
Sauermann U
机构信息
Arbeitsgruppe Primatengenetic, Deutsches Primatenzentrum, Göttingen, Germany.
出版信息
Tissue Antigens. 1998 Dec;52(6):550-7. doi: 10.1111/j.1399-0039.1998.tb03086.x.
The DQA1 and DQB1 alleles of 258 rhesus monkeys (Macaca mulatta) of different origin were typed by PCR-RFLP. Five novel MamuDQA1 and five novel -DQB1 alleles were detected and 15 Mamu-DQA1-DQB1 haplotypes were identified. Haplotype analysis confirmed the conservation of the DQA1*01-DQB1 *06 haplotypes in evolution. The most conspicuous finding was the tight linkage between the Mamu-DQA1 and -DQB1 alleles. Almost in every case the Mamu-DQA1 allele was linked to only one particular Mamu-DQB1 allele. Although there also are constraints in the formation of DQ haplotypes in humans, such tight linkages are not observed. These findings support the hypothesis of some kind of co-evolution between DQA1 and DQB1 alleles and may reflect a stronger force of natural selection in macaques than in humans.
采用聚合酶链反应-限制性片段长度多态性方法（PCR-RFLP）对258只不同来源的恒河猴（猕猴）的DQA1和DQB1等位基因进行分型。检测到5个新的MamuDQA1等位基因和5个新的-DQB1等位基因，并鉴定出15种Mamu-DQA1-DQB1单倍型。单倍型分析证实了DQA1*01-DQB1 *06单倍型在进化过程中的保守性。最显著的发现是Mamu-DQA1和-DQB1等位基因之间的紧密连锁。几乎在每种情况下，Mamu-DQA1等位基因仅与一种特定的Mamu-DQB1等位基因连锁。虽然人类DQ单倍型的形成也存在限制，但未观察到如此紧密的连锁。这些发现支持了DQA1和DQB1等位基因之间某种共同进化的假说，并且可能反映出猕猴比人类具有更强的自然选择力。
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