铜绿假单胞菌脂多糖的异质性。

Heterogeneity of the lipopolysaccharide from Pseudomonas aeruginosa.

作者信息

Chester I R, Meadow P M

出版信息

Eur J Biochem. 1975 Oct 15;58(2):273-82. doi: 10.1111/j.1432-1033.1975.tb02373.x.

DOI:10.1111/j.1432-1033.1975.tb02373.x

Abstract

Lipopolysaccharide isolated from pseudomonas aeruginosa PAC1 and its phage-resistant mutant was degraded by mild acid hydrolysis into lipid A and three major polysaccharide-containing fractions which were separated on Sephadex G-75. The low-molecular-weight fraction contained glucose, rhamnose, heptose, galactosamine, alanine and phosphate. The higher-molecular-weight fractions consisted mainly of glucose, rhamnose and glucosamine together with amino compounds. Alkaline degradation of the lipopolysaccharide produced at least four different species each of which contained a low-molecular-weight polysaccharide similar if not identical to that produced by acid hydrolysis. Under certain growth conditions an abnormal lipopolysaccharide was produced which was defective in the low-molecular-weight polysaccharide and contained mainly high-molecular-weight material. Strains of different serotype yielded lipopolysaccharides which also exhibited heterogeneity but contained a low-molecular-weight polysaccharide similar to that obtained from strain PAC1 and PAC1R. It is suggested that each strain of P. aeruginosa may produce several lipopolysaccharides each containing a polysaccharide common to all. The relative proportions of the various lipopolysaccharides may be changed by growth conditions.

摘要

从铜绿假单胞菌PAC1及其噬菌体抗性突变体中分离出的脂多糖，通过温和酸水解降解为脂质A和三个主要的含多糖级分，这些级分在葡聚糖G-75上进行了分离。低分子量级分含有葡萄糖、鼠李糖、庚糖、半乳糖胺、丙氨酸和磷酸盐。较高分子量级分主要由葡萄糖、鼠李糖和葡糖胺以及氨基化合物组成。脂多糖的碱性降解产生了至少四种不同的物质，每种物质都含有一种与酸水解产生的低分子量多糖相似（如果不是相同的话）的低分子量多糖。在某些生长条件下，会产生一种异常的脂多糖，其低分子量多糖有缺陷，主要含有高分子量物质。不同血清型的菌株产生的脂多糖也表现出异质性，但含有一种与从菌株PAC1和PAC1R获得的低分子量多糖相似的低分子量多糖。有人提出，铜绿假单胞菌的每个菌株可能产生几种脂多糖，每种脂多糖都含有一种所有脂多糖共有的多糖。各种脂多糖的相对比例可能会因生长条件而改变。