Palittapongarnpim P, Rienthong S, Panbangred W
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Tuber Lung Dis. 1993 Jun;74(3):204-7. doi: 10.1016/0962-8479(93)90013-N.
Strain characterization of Mycobacterium tuberculosis has been based mainly on mycobacteriophage typing or chromosomal DNA restriction fragment analysis. In this study 10 randomly selected EcoRI chromosomal DNA fragments of M. tuberculosis H37RV were labelled with digoxigenin and used to probe the Southern blot preparation of EcoRI or BstEII digested chromosomal DNA of clinical isolates of M. tuberculosis. 2 probes were able to reveal restriction fragment length polymorphism. Each of the probes divided 15 pulmonary and 6 cerebrospinal fluid (CSF) isolates of M. tuberculosis into 3 groups and combination of both probes divided them into 4 groups. All of the 6 CSF isolates belonged to 1 group while only 5 of the 15 pulmonary isolates belonged to the same group. Work is continuing in order to characterize the nature of the probe and confirm the results in a larger population.
结核分枝杆菌的菌株鉴定主要基于分枝杆菌噬菌体分型或染色体DNA限制性片段分析。在本研究中，结核分枝杆菌H37RV的10个随机选择的EcoRI染色体DNA片段用地高辛标记，并用于探测经EcoRI或BstEII消化的结核分枝杆菌临床分离株染色体DNA的Southern杂交制备物。2个探针能够揭示限制性片段长度多态性。每个探针将15株肺结核分枝杆菌分离株和6株结核性脑膜炎脑脊液分枝杆菌分离株分为3组，两个探针组合则将它们分为4组。6株脑脊液分离株均属于同一组，而15株肺结核分离株中只有5株属于同一组。为了鉴定探针的性质并在更大的群体中证实结果，研究仍在继续。
