Schoen S W, Graybiel A M
Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge 02139.
J Comp Neurol. 1993 Jul 22;333(4):578-96. doi: 10.1002/cne.903330410.
The glycoprotein 5'-nucleotidase is a cell surface phosphatase and represents a new marker for striosomes in the adult rat caudoputamen. We report here on its developmental expression in the rat and mouse striatum, and show an unexpected converse 5'-nucleotidase chemoarchitecture of the caudoputamen in these closely related species. In the rat, 5'-nucleotidase activity was first visible as neuropil staining in tyrosine hydroxylase-positive dopamine islands of the midstriatum on postnatal day 1, and by the end of the first postnatal week, 5'-nucleotidase-positive dopamine islands also appeared rostrally. This compartmental pattern persisted thereafter, so that in adult animals, in all but the caudal caudoputamen, zones of enhanced 5'-nucleotidase staining were restricted to calbindin-D28k-poor striosomes. Weak 5'-nucleotidase activity also emerged in the matrix. In striking contrast, in the mouse striatum, enhanced 5'-nucleotidase activity was preferentially associated with extrastriosomal tissue. Enzymatic reaction first appeared on embryonic day 18, and developed over the first postnatal week into a mosaic pattern in which the matrix was stained but the dopamine islands were unstained. The matrix staining itself was heterogeneous. After the second postnatal week, most of the caudoputamen was stained, and in adult mice only rostral striosomes expressed low 5'-nucleotidase activity. We conclude that in rats, 5'-nucleotidase represents one of the few substances that maintains a preferential dopamine island/striosome distribution during striatal development. In mice, 5'-nucleotidase activity is expressed preferentially in the matrix during development, and its compartmental pattern is gradually lost with maturation, except very rostrally. These findings do not suggest an instructive role of the enzyme in striatal compartment formation in either species, but do suggest the possibility that 5'-nucleotidase contributes to the differentiation of striatal compartments during development.
糖蛋白5'-核苷酸酶是一种细胞表面磷酸酶，是成年大鼠尾壳核中纹状体小体的一种新标记物。我们在此报告其在大鼠和小鼠纹状体中的发育表达情况，并显示在这些密切相关的物种中，尾壳核的5'-核苷酸酶化学结构存在意想不到的差异。在大鼠中，5'-核苷酸酶活性最初在出生后第1天以神经毡染色的形式出现在中纹状体酪氨酸羟化酶阳性的多巴胺岛中，到出生后第一周结束时，5'-核苷酸酶阳性的多巴胺岛也出现在前端。此后，这种分区模式持续存在，因此在成年动物中，除了尾侧尾壳核外，5'-核苷酸酶染色增强的区域仅限于钙结合蛋白-D28k含量低的纹状体小体。基质中也出现了微弱的5'-核苷酸酶活性。与之形成鲜明对比的是，在小鼠纹状体中，5'-核苷酸酶活性增强主要与纹状体小体外部组织相关。酶反应最早出现在胚胎第18天，并在出生后第一周发展成一种镶嵌模式，其中基质被染色而多巴胺岛未被染色。基质染色本身是不均匀的。出生后第二周后，大部分尾壳核被染色，在成年小鼠中，只有前端纹状体小体表达低水平的5'-核苷酸酶活性。我们得出结论，在大鼠中，5'-核苷酸酶是在纹状体发育过程中维持多巴胺岛/纹状体小体优先分布的少数物质之一。在小鼠中，5'-核苷酸酶活性在发育过程中优先在基质中表达，并且其分区模式随着成熟逐渐消失，除了非常前端的区域。这些发现并不表明该酶在任何一个物种的纹状体分区形成中具有指导作用，但确实表明5'-核苷酸酶在发育过程中有助于纹状体分区的分化。
