Shu S Y, Bao X M, Zhang C, Li S X, Chan W Y, Yew D
Department of Neurobiology, Institute for Neuroscience of First Military Medical University, Zhu-Jiang Hospital, Guangzhou, China.
Neurochem Res. 2000 Feb;25(2):231-7. doi: 10.1023/a:1007523520251.
A new subdivision, the "marginal division" (MrD), was discovered at the caudal border of the striatum and surrounds the rostral edge of the globus pallidus in the rat brain in our previous studies. The neuronal somata of the MrD are mostly fusiform in shape with their long axes lining dorsoventrally. The MrD is more densely filled with substance P (SP)-, Leucine-enkephalin (L-Enk)-, dynorphin B-, neurotensin-, somatostatin- and cholecystokinin (CCK)-immunoreactive fibers and terminal-like structures than the rest of the striatum. The MrD was confirmed in the cat neostriatum as well. The present study intended to explore whether the MrD exists in the monkey neostriatum (putamen) with Nissl, histochemical and immunohistochemical methods. A band of fusiform neurons were obviously identified at the caudomedial edge of the putamen. These neurons lie outside the lateral medullary lamina and indirectly surround the rostrolateral border of the globus pallidus. The abundance of SP-, L-Enk-, neuropeptide Y-, CCK-, dopamine- and serotonin-positive fibers and terminal-like structures with a few positive fusiform neurons accumulating at the caudomedial border of the putamen obviously distinguishes this zone from the rest of neostriatum and globus pallidus. The acetylcholinesterase (AChE) positive and nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) containing fusiform neurons are distinctly visualized in the same zone. The morphological figure and the location of these neurons, and the histochemical and immunohistochemical characteristics of this area coincide well with those of the MrD in the rat and cat striatum. This study thus convincingly identifies the existence of the MrD in the monkey neostriatum. It is fairly asserted that the MrD is a universal structure in the mammalian brain.
在我们之前的研究中，在大鼠脑内纹状体的尾侧边界发现了一个新的分区，即“边缘区”（MrD），它环绕苍白球的嘴侧边缘。MrD的神经元胞体大多呈梭形，其长轴沿背腹方向排列。与纹状体的其他部分相比，MrD中富含P物质（SP）、亮氨酸脑啡肽（L-Enk）、强啡肽B、神经降压素、生长抑素和胆囊收缩素（CCK）免疫反应性纤维及终末样结构。在猫的新纹状体中也证实了MrD的存在。本研究旨在用尼氏染色、组织化学和免疫组织化学方法探索MrD是否存在于猴的新纹状体（壳核）中。在壳核的尾内侧边缘明显识别出一条梭形神经元带。这些神经元位于外侧髓板之外，间接环绕苍白球的嘴外侧边界。壳核尾内侧边界处大量的SP、L-Enk、神经肽Y、CCK、多巴胺和5-羟色胺阳性纤维及终末样结构，以及少数阳性梭形神经元的聚集，明显将该区域与新纹状体和苍白球的其他部分区分开来。在同一区域可清晰看到乙酰胆碱酯酶（AChE）阳性和含烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）的梭形神经元。这些神经元的形态、位置以及该区域的组织化学和免疫组织化学特征与大鼠和猫纹状体中的MrD非常吻合。因此，本研究令人信服地证实了猴新纹状体中存在MrD。可以确切地说，MrD是哺乳动物脑中的一个普遍结构。
