Dalton S, Marais R, Wynne J, Treisman R
Transcription Laboratory, Imperial Cancer Research Fund, London, U.K.
Philos Trans R Soc Lond B Biol Sci. 1993 Jun 29;340(1293):325-32. doi: 10.1098/rstb.1993.0074.
Many genes which are regulated by growth factors contain a common regulatory element, the serum response element (SRE). Activation of transcription by the SRE involves a ternary complex formed between a ubiquitous factor, serum response factor (SRF), and a second protein, p62/TCF. We used a yeast genetic screen to isolate cDNAs encoding a protein, SAP-1, with the DNA binding properties of p62/TCF. The SAP-1 sequence contains three regions of homology to the previously uncharacterized Elk-1 protein, which also acts as an SRF accessory protein. Only two of these regions are required for cooperative interactions with SRF in the ternary complex. The third contains several conserved sites for the MAP kinases, whose activity is regulated in response to growth factor stimulation. We discuss the potential role of these proteins in regulation of the c-fos SRE.
许多受生长因子调控的基因都含有一个共同的调控元件，即血清反应元件（SRE）。SRE介导的转录激活涉及一种三元复合物的形成，该复合物由一种普遍存在的因子——血清反应因子（SRF）和另一种蛋白质p62/TCF组成。我们利用酵母遗传筛选技术分离出了编码一种蛋白质SAP-1的cDNA，该蛋白质具有p62/TCF的DNA结合特性。SAP-1序列包含与先前未鉴定的Elk-1蛋白同源的三个区域，Elk-1蛋白也作为SRF辅助蛋白发挥作用。在三元复合物中，与SRF协同相互作用仅需要其中两个区域。第三个区域包含几个丝裂原活化蛋白激酶（MAP激酶）的保守位点，其活性受生长因子刺激的调控。我们讨论了这些蛋白质在c-fos SRE调控中的潜在作用。
