Ohno T, Imai A, Furui T, Takahashi K, Tamaya T
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Japan.
Am J Obstet Gynecol. 1993 Sep;169(3):605-10. doi: 10.1016/0002-9378(93)90630-2.
The purpose of this study was to investigate the expression of gonadotropin-releasing hormone messenger ribonucleic acid and the presence of gonadotropin-releasing hormone in human ovarian carcinoma known to have gonadotropin-releasing hormone binding sites and to be affected by gonadotropin-releasing hormone analog.
Human ovarian carcinomas surgically removed and human ovarian carcinoma cell lines were examined. Gonadotropin-releasing hormone was determined by a radioimmunoassay and a bioassay. Gonadotropin-releasing hormone messenger ribonucleic acid was determined by reverse transcription polymerase chain reaction using oligonucleotide primers synthesized according to the published human gonadotropin-releasing hormone sequence.
Gonadotropin-releasing hormone was shown to be present in extracts of ovarian mucinous cystadenocarcinoma sample (0.8 +/- 0.12 pg/mg of protein) and ovarian adenocarcinoma cell line SK-OV3 (0.92 +/- 0.17 pg/mg of protein) but not in the normal ovary and placenta. Two of two extract samples from individual cases evoked dose-dependent phosphoinositide breakdown in rat granulosa cells similar to that caused by authentic gonadotropin-releasing hormone. Gonadotropin-releasing hormone messenger ribonucleic acid was detected in two of two mucinous cystadenocarcinoma specimens, one of one serous cystadenocarcinoma, and SK-OV3 cells but not in the dysgerminoma, mucinous cystadenoma, and normal ovary and placenta.
The demonstration of gonadotropin-releasing hormone and its messenger ribonucleic acid raises the possibility that gonadotropin-releasing hormone may play an autocrine regulatory role in the growth of ovarian carcinoma.
本研究旨在调查促性腺激素释放激素信使核糖核酸的表达情况，以及在已知具有促性腺激素释放激素结合位点且受促性腺激素释放激素类似物影响的人卵巢癌中促性腺激素释放激素的存在情况。
对手术切除的人卵巢癌和人卵巢癌细胞系进行检查。通过放射免疫测定法和生物测定法测定促性腺激素释放激素。使用根据已发表的人促性腺激素释放激素序列合成的寡核苷酸引物，通过逆转录聚合酶链反应测定促性腺激素释放激素信使核糖核酸。
促性腺激素释放激素在卵巢黏液性囊腺癌样本提取物（0.8±0.12 pg/mg蛋白质）和卵巢腺癌细胞系SK-OV3（0.92±0.17 pg/mg蛋白质）中被检测到，但在正常卵巢和胎盘中未检测到。来自个别病例的两个提取物样本中的两个在大鼠颗粒细胞中引起了剂量依赖性磷酸肌醇分解，类似于由 authentic促性腺激素释放激素引起的分解。在两个黏液性囊腺癌标本中的两个、一个浆液性囊腺癌标本以及SK-OV3细胞中检测到促性腺激素释放激素信使核糖核酸，但在无性细胞瘤、黏液性囊腺瘤以及正常卵巢和胎盘中未检测到。
促性腺激素释放激素及其信使核糖核酸的证实增加了促性腺激素释放激素可能在卵巢癌生长中发挥自分泌调节作用 的可能性。
