Tracqui A, Kintz P, Mangin P
Institut de Médecine Légale, Faculté de Médecine de Strasbourg, France.
J Chromatogr. 1993 Jun 23;616(1):95-103. doi: 10.1016/0378-4347(93)80476-k.
A high-performance liquid chromatographic assay with diode-array detection has been developed for the toxicological screening of the newly developed non-benzodiazepine hypnotics and anxiolytics zopiclone, zolpidem, suriclone and alpidem. After single-step liquid-liquid extraction of plasma at pH 9.5 using chloroform-2-propanol-n-heptane (60:14:26, v/v), the substances are separated on a Nova-Pak C18 4-microns column (300 mm x 3.9 mm, I.D.), with methanol-tetrahydrofuran-pH 2.6 phosphate buffer (65:5:30, v/v) as the mobile phase (flow-rate 0.8 ml/min). Full ultraviolet spectra from 200 to 400 nm are recorded on-line during the entire analysis and may be automatically compared to spectra stored in a library. The retention times of the four drugs are 4.05 min (zopiclone), 4.66 min (zolpidem), 6.74 min (suriclone) and 10.97 min (alpidem). The analysis is performed in 15 min. The method is simple, rapid and highly specific. It is the first assay to be described for convenient screening of cyclopyrrolones and imidazopyridines.
已开发出一种带二极管阵列检测的高效液相色谱分析法，用于对新开发的非苯二氮䓬类催眠药和抗焦虑药佐匹克隆、唑吡坦、舒利克隆和阿吡坦进行毒理学筛查。在pH 9.5条件下，使用氯仿 - 2 - 丙醇 - 正庚烷（60:14:26，v/v）对血浆进行单步液 - 液萃取后，这些物质在Nova - Pak C18 4微米柱（300毫米×3.9毫米，内径）上分离，以甲醇 - 四氢呋喃 - pH 2.6磷酸盐缓冲液（65:5:30，v/v）作为流动相（流速0.8毫升/分钟）。在整个分析过程中在线记录200至400纳米的全紫外光谱，并可自动与存储在库中的光谱进行比较。这四种药物的保留时间分别为4.05分钟（佐匹克隆）、4.66分钟（唑吡坦）、6.74分钟（舒利克隆）和10.97分钟（阿吡坦）。分析在15分钟内完成。该方法简单、快速且具有高度特异性。它是首个用于方便筛查环吡咯酮类和咪唑吡啶类药物的分析方法。
