Lew J Y, Heidelberger M, Griffiths M
Int J Pept Protein Res. 1975;7(4):289-93. doi: 10.1111/j.1399-3011.1975.tb02443.x.
The portion of echnida sublingual glycoproteins which could be solubilized was fractionated by electrophoresis in polyacrylamide gel and by filtration on a column of Sephadex G-100. Analyses for carbohydrate and amino acid are given and are compared with those of the submaxillary mucins of cattle, dog, pig and sheep. The molar ratio of galactosamine to sialic acid was roughly 1:1 in the echidna fractions, the sialic acid was N-acetyl-neuraminic acid, and there was very little neutral sugar. Proline was relatively high in amount, and isoleucine, half-cystine, methionine, leucine, tyrosine, and phenylalanine were absent or present to a very small extent. The material was weakly antigenic in the rabbit.
针鼹鼠舌下糖蛋白中可溶解的部分通过聚丙烯酰胺凝胶电泳和在葡聚糖G - 100柱上过滤进行分级分离。给出了碳水化合物和氨基酸的分析结果，并与牛、狗、猪和羊的颌下粘蛋白的分析结果进行了比较。针鼹鼠分级部分中半乳糖胺与唾液酸的摩尔比约为1:1，唾液酸为N - 乙酰神经氨酸，中性糖含量很少。脯氨酸含量相对较高，异亮氨酸、半胱氨酸、蛋氨酸、亮氨酸、酪氨酸和苯丙氨酸不存在或含量极少。该物质在兔体内具有弱抗原性。
