Hou L T
Department of Periodontology, School of Dentistry, National Taiwan University, Taipei, R.O.C.
J Formos Med Assoc. 1993 Apr;92(4):367-72.
The purpose of the present study was to compare protein and matrix molecular (collagen and fibronectin) metabolism among fibroblasts cultured from two types of hyperplastic gingiva and normal tissues. Cell cultures were obtained from tissue explants of normal gingiva, the periodontal ligament (PDL), and phenytoin-induced and idiopathic fibromatotic gingiva. The synthetic capacity of matrix proteins of each isolated fibroblast type was studied by metabolic labeling (3H-proline), biochemical purification and characterization. Our results showed that fibroblasts derived from phenytoin-induced gingival hyperplasia synthesized a greater amount of total protein and collagen than the rest of the cell types, based on both the amount of total protein and protein/micrograms DNA. In contrast, fibroblasts obtained from idiopathic gingival fibromatosis exhibited a similar potential for both protein and collagen synthesis as normal gingival and PDL fibroblasts. Normal PDL fibroblasts displayed a significantly higher degree of collagen synthesis than normal gingival fibroblasts; however, no difference in protein production between them was noted. A few variations in fibronectin synthesis/microgram DNA among various fibroblasts from normal and hyperplastic tissues were noted. PDL fibroblasts seemed to produce a greater amount of fibronectin than all of the other cell types. Interestingly, fibroblasts obtained from idiopathic gingival fibromatosis showed a higher DNA content than other fibroblasts, although a decrease in synthesized fibronectin/microgram DNA was noted. In conclusion, the pathogenic mechanism of phenytoin-induced hyperplasia and idiopathic gingival fibromatosis may be different due to their distinct profile of cell proliferation and matrix protein synthesis.
本研究的目的是比较从两种增生性牙龈组织和正常组织培养的成纤维细胞之间蛋白质和基质分子（胶原蛋白和纤连蛋白）的代谢情况。细胞培养物取自正常牙龈、牙周膜（PDL）、苯妥英钠诱导的和特发性纤维瘤样牙龈的组织外植体。通过代谢标记（3H-脯氨酸）、生化纯化和表征研究了每种分离的成纤维细胞类型的基质蛋白合成能力。我们的结果表明，基于总蛋白量和蛋白/微克DNA量，从苯妥英钠诱导的牙龈增生中获得的成纤维细胞比其他细胞类型合成了更多的总蛋白和胶原蛋白。相比之下，从特发性牙龈纤维瘤病获得的成纤维细胞在蛋白质和胶原蛋白合成方面表现出与正常牙龈和成纤维细胞相似的潜力。正常的牙周膜成纤维细胞显示出比正常牙龈成纤维细胞更高程度的胶原蛋白合成；然而，未观察到它们之间蛋白质产生的差异。注意到来自正常和增生组织的各种成纤维细胞之间在纤连蛋白合成/微克DNA方面存在一些差异。牙周膜成纤维细胞似乎比所有其他细胞类型产生更多的纤连蛋白。有趣的是，从特发性牙龈纤维瘤病获得的成纤维细胞显示出比其他成纤维细胞更高的DNA含量，尽管观察到合成的纤连蛋白/微克DNA有所减少。总之，由于苯妥英钠诱导的增生和特发性牙龈纤维瘤病在细胞增殖和基质蛋白合成方面具有不同的特征，其致病机制可能不同。
